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primary topic: https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/20217

https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/20217
type: AcademicArticle
type: Article
title: Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study
abstract: Background: Adipose tissue-derived mesenchymal stromal cells (AT-MSCs) are frequently used to treat equine tendinopathies. Up to now, knowledge about the fate of autologous AT-MSCs after intralesional injection into equine superficial digital flexor tendons (SDFTs) is very limited. The purpose of this study was to monitor the presence of intralesionally injected autologous AT-MSCs labelled with superparamagnetic iron oxide (SPIO) nanoparticles and green fluorescent protein (GFP) over a staggered period of 3 to 9 weeks with standing magnetic resonance imaging (MRI) and histology. Methods: Four adult warmblood horses received a unilateral injection of 10 × 106 autologous AT-MSCs into surgically created front-limb SDFT lesions. Administered AT-MSCs expressed lentivirally transduced reporter genes for GFP and were co-labelled with SPIO particles in three horses. The presence of AT-MSCs in SDFTs was evaluated by repeated examinations with standing low-field MRI in two horses and post-mortem in all horses with Prussian blue staining, fluorescence microscopy and with immunofluorescence and immunohistochemistry using anti-GFP antibodies at 3, 5, 7 and 9 weeks after treatment. Results: AT-MSCs labelled with SPIO particles were detectable in treated SDFTs during each MRI in T2*- and T1-weighted sequences until the end of the observation period. Post-mortem examinations revealed that all treated tendons contained high numbers of SPIO- and GFP-labelled cells. Conclusions: Standing low-field MRI has the potential to track SPIO-labelled AT-MSCs successfully. Histology, fluorescence microscopy, immunofluorescence and immunohistochemistry are efficient tools to detect labelled AT-MSCs after intralesional injection into surgically created equine SDFT lesions. Intralesional injection of 10 × 106 AT-MSCs leads to the presence of high numbers of AT-MSCs in and around surgically created tendon lesions for up to 9 weeks. Integration of injected AT-MSCs into healing tendon tissue is an es…
date: 2016
volume: 7
issue: 21
is part of: Stem Cell Research & Therapy
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185163
has document: https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185165
has document: https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185166
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has document: https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185169
subject: 570 Biowissenschaften, Biologie

https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185163
type: Document
label: Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (PDF)
has file: 13287_2016_Article_281.pdf
has part: 13287_2016_Article_281.pdf

BioMed Central
type: Organization

Stem Cell Research & Therapy
type: Collection
issn: 17576512

Kathrin Mundle
type: Person
given name: Kathrin
family name: Mundle

Maren Hellige
type: Person
given name: Maren
family name: Hellige

Thomas Skutella
type: Person
given name: Thomas
family name: Skutella

Florian Geburek
type: Person
given name: Florian
family name: Geburek

Peter M. Stadler
type: Person
given name: Peter M.
family name: Stadler

Ulrich Walliser
type: Person
given name: Ulrich
family name: Walliser

René van Weeren
type: Person
given name: René
family name: van Weeren

Hans T. M. van Schie
type: Person
given name: Hans T. M.
family name: van Schie

Sabine Conrad
type: Person
given name: Sabine
family name: Conrad

https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185165
type: Document
label: Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)
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https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185166
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label: Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)
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https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185167
type: Document
label: Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)
is version of: https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185163
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https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185168
type: Document
label: Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)
is version of: https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185163
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https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/185169
type: Document
label: Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)
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https://archiv.ub.uni-heidelberg.de/volltextserver/20217/
title: HTML Summary of #20217 Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study
format: text/html
primary topic: https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/20217

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en