<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study"^^ . "Background: Adipose tissue-derived mesenchymal stromal cells (AT-MSCs) are frequently used to treat equine tendinopathies. Up to now, knowledge about the fate of autologous AT-MSCs after intralesional injection into equine superficial digital flexor tendons (SDFTs) is very limited. The purpose of this study was to monitor the presence of intralesionally injected autologous AT-MSCs labelled with superparamagnetic iron oxide (SPIO) nanoparticles and green fluorescent protein (GFP) over a staggered period of 3 to 9 weeks with standing magnetic resonance imaging (MRI) and histology. Methods: Four adult warmblood horses received a unilateral injection of 10 × 106 autologous AT-MSCs into surgically created front-limb SDFT lesions. Administered AT-MSCs expressed lentivirally transduced reporter genes for GFP and were co-labelled with SPIO particles in three horses. The presence of AT-MSCs in SDFTs was evaluated by repeated examinations with standing low-field MRI in two horses and post-mortem in all horses with Prussian blue staining, fluorescence microscopy and with immunofluorescence and immunohistochemistry using anti-GFP antibodies at 3, 5, 7 and 9 weeks after treatment. Results: AT-MSCs labelled with SPIO particles were detectable in treated SDFTs during each MRI in T2*- and T1-weighted sequences until the end of the observation period. Post-mortem examinations revealed that all treated tendons contained high numbers of SPIO- and GFP-labelled cells. Conclusions: Standing low-field MRI has the potential to track SPIO-labelled AT-MSCs successfully. Histology, fluorescence microscopy, immunofluorescence and immunohistochemistry are efficient tools to detect labelled AT-MSCs after intralesional injection into surgically created equine SDFT lesions. Intralesional injection of 10 × 106 AT-MSCs leads to the presence of high numbers of AT-MSCs in and around surgically created tendon lesions for up to 9 weeks. Integration of injected AT-MSCs into healing tendon tissue is an essential pathway after intralesional administration. Injection techniques have to be chosen deliberately to avoid reflux of the cell substrate injected. In vivo low-field MRI may be used as a non-invasive tool to monitor homing and engraftment of AT-MSCs in horses with tendinopathy of the SDFT."^^ . "2016" . . . "7" . "21" . . "BioMed Central"^^ . . . "Stem Cell Research & Therapy"^^ . . . "17576512" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Ulrich"^^ . "Walliser"^^ . "Ulrich Walliser"^^ . . "Florian"^^ . "Geburek"^^ . "Florian Geburek"^^ . . "Kathrin"^^ . "Mundle"^^ . "Kathrin Mundle"^^ . . "Peter M."^^ . "Stadler"^^ . "Peter M. Stadler"^^ . . "Thomas"^^ . "Skutella"^^ . "Thomas Skutella"^^ . . "Sabine"^^ . "Conrad"^^ . "Sabine Conrad"^^ . . "René"^^ . "van Weeren"^^ . "René van Weeren"^^ . . "Hans T. M."^^ . "van Schie"^^ . "Hans T. M. van Schie"^^ . . "Maren"^^ . "Hellige"^^ . "Maren Hellige"^^ . . . . . . "Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (PDF)"^^ . . . "13287_2016_Article_281.pdf"^^ . . . "Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)"^^ . . . . . . "small.jpg"^^ . . . "Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #20217 \n\nTracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .