eprintid: 20217
rev_number: 11
eprint_status: archive
userid: 1589
dir: disk0/00/02/02/17
datestamp: 2016-03-01 08:16:39
lastmod: 2016-03-01 10:45:12
status_changed: 2016-03-01 08:16:39
type: article
metadata_visibility: show
creators_name: Geburek, Florian
creators_name: Mundle, Kathrin
creators_name: Conrad, Sabine
creators_name: Hellige, Maren
creators_name: Walliser, Ulrich
creators_name: van Schie, Hans T. M.
creators_name: van Weeren, René
creators_name: Skutella, Thomas
creators_name: Stadler, Peter M.
title: Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study
subjects: 570
divisions: 53100
abstract: Background: Adipose tissue-derived mesenchymal stromal cells (AT-MSCs) are frequently used to treat equine tendinopathies. Up to now, knowledge about the fate of autologous AT-MSCs after intralesional injection into equine superficial digital flexor tendons (SDFTs) is very limited. The purpose of this study was to monitor the presence of intralesionally injected autologous AT-MSCs labelled with superparamagnetic iron oxide (SPIO) nanoparticles and green fluorescent protein (GFP) over a staggered period of 3 to 9 weeks with standing magnetic resonance imaging (MRI) and histology.   Methods: Four adult warmblood horses received a unilateral injection of 10 × 106 autologous AT-MSCs into surgically created front-limb SDFT lesions. Administered AT-MSCs expressed lentivirally transduced reporter genes for GFP and were co-labelled with SPIO particles in three horses. The presence of AT-MSCs in SDFTs was evaluated by repeated examinations with standing low-field MRI in two horses and post-mortem in all horses with Prussian blue staining, fluorescence microscopy and with immunofluorescence and immunohistochemistry using anti-GFP antibodies at 3, 5, 7 and 9 weeks after treatment.   Results: AT-MSCs labelled with SPIO particles were detectable in treated SDFTs during each MRI in T2*- and T1-weighted sequences until the end of the observation period. Post-mortem examinations revealed that all treated tendons contained high numbers of SPIO- and GFP-labelled cells.   Conclusions: Standing low-field MRI has the potential to track SPIO-labelled AT-MSCs successfully. Histology, fluorescence microscopy, immunofluorescence and immunohistochemistry are efficient tools to detect labelled AT-MSCs after intralesional injection into surgically created equine SDFT lesions. Intralesional injection of 10 × 106 AT-MSCs leads to the presence of high numbers of AT-MSCs in and around surgically created tendon lesions for up to 9 weeks. Integration of injected AT-MSCs into healing tendon tissue is an essential pathway after intralesional administration. Injection techniques have to be chosen deliberately to avoid reflux of the cell substrate injected. In vivo low-field MRI may be used as a non-invasive tool to monitor homing and engraftment of AT-MSCs in horses with tendinopathy of the SDFT.
date: 2016
publisher: BioMed Central
id_scheme: DOI
id_number: http://dx.doi.org/10.1186/s13287-016-0281-8
ppn_swb: 165531274X
own_urn: urn:nbn:de:bsz:16-heidok-202171
language: eng
bibsort: GEBUREKFLOTRACKINGOF2016
full_text_status: public
publication: Stem Cell Research & Therapy
volume: 7
number: 21
place_of_pub: London
pagerange: 1-12
issn: 1757-6512
citation:   Geburek, Florian ; Mundle, Kathrin ; Conrad, Sabine ; Hellige, Maren ; Walliser, Ulrich ; van Schie, Hans T. M. ; van Weeren, René ; Skutella, Thomas ; Stadler, Peter M.  (2016) Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions - a pilot study.  Stem Cell Research & Therapy, 7 (21).  pp. 1-12.  ISSN 1757-6512     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/20217/1/13287_2016_Article_281.pdf