<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Functional characterization of the interaction of Paraxial protocadherin (PAPC) and Nemo like kinase1 (Nlk1) during Xenopus gastrulation"^^ . "During the development of an organism, gastrulation is a critical step laying the foundation of organogenesis. Cells in gastrulating embryos undergo a variety of coordinated cell movements to establish the three germ layers, ectoderm, mesoderm and endoderm. In this, concerted cell movements are orchestrated by multiple signaling pathways, but most importantly by different Wnt signaling cascades, such as the non-canonical Wnt/planar cell polarity (PCP) pathway. Paraxial protocadherin (PAPC) is a central player during Xenopus gastrulation regulating cell adhesion, polarization and the Wnt/PCP pathway. However, the mechanism of PAPC mediated signaling is not entirely understood. In this work I could show for the first time that PAPC interacts with Nemo like kinase 1 (Nlk1), an atypical Serine/Threonine MAP kinase and a known inhibitor of the canonical Wnt/β-catenin pathway. The interaction of Nlk1 with PAPC is mediated by the intracellular domain of PAPC, which triggers recruitment of Nlk1 to the plasma membrane. I further provide evidence that binding to each other causes a post-translational stabilization of both proteins, by inhibition of ubiquitination. Using a kinase negative mutant of Nlk1, I could show that this is independent of its kinase activity. At the same time, mutations of the putative Nlk1 phosphorylation sites in PAPC indicated that phosphorylation of these sites is required for Nlk1 mediated stabilization of PAPC. Notably, I could demonstrate that Nlk1, probably via its interaction with PAPC, is required for the regulation of Wnt/PCP signaling and gastrulation during early Xenopus development, in addition to its function in Wnt/β-catenin signaling. Interestingly, I found that the kinase activity of Nlk1, while required for canonical Wnt modulation, is dispensable for its role in Wnt/PCP signaling and regulation of gastrulation. Based on my results I propose that during Xenopus development Nlk1 is a versatile modulator of the Wnt/β-catenin and Wnt/PCP pathways. Moreover, I suggest that Nlk1 influences gastrulation movements by stabilizing the Wnt/PCP component PAPC."^^ . "2016" . . . . . . . "Rahul"^^ . "Kumar"^^ . "Rahul Kumar"^^ . . . . . . "Functional characterization of the interaction of Paraxial protocadherin (PAPC) and Nemo like kinase1 (Nlk1) during Xenopus gastrulation (Other)"^^ . . . . . . "Functional characterization of the interaction of Paraxial protocadherin (PAPC) and Nemo like kinase1 (Nlk1) during Xenopus gastrulation (Other)"^^ . . . . . . "Functional characterization of the interaction of Paraxial protocadherin (PAPC) and Nemo like kinase1 (Nlk1) during Xenopus gastrulation (PDF)"^^ . . . "Rahul AG ST thesis..pdf"^^ . . . "Functional characterization of the interaction of Paraxial protocadherin (PAPC) and Nemo like kinase1 (Nlk1) during Xenopus gastrulation (Other)"^^ . . . . . . "Functional characterization of the interaction of Paraxial protocadherin (PAPC) and Nemo like kinase1 (Nlk1) during Xenopus gastrulation (Other)"^^ . . . . . . "Functional characterization of the interaction of Paraxial protocadherin (PAPC) and Nemo like kinase1 (Nlk1) during Xenopus gastrulation (Other)"^^ . . . . . "HTML Summary of #20565 \n\nFunctional characterization of the interaction of Paraxial protocadherin (PAPC) and Nemo like kinase1 (Nlk1) during Xenopus gastrulation\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .