TY  - JOUR
ID  - heidok20744
PB  - BioMed Central
TI  - Serum dihydroxyacetone kinase peptide m/z 520.3 as predictor of disease severity in patients with compensated chronic hepatitis B
N2  - Background & aim: Due to known limitations of liver biopsy, reliable non-invasive serum biomarkers for chronic liver diseases are needed. We performed serum peptidomics for such investigation in compensated chronic hepatitis B (CHB) patients.   Methods: Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) was used to identify differentially expressed peptides in sera from 40 CHB patients (20 with S0G0-S1G1 and 20 with S3G3-S4G4). Ion pair quantification from differentially expressed peptides in a validation set of sera from 86 CHB patients was done with multiple reaction monitoring (MRM).   Results: 21 differentially represented peptide peaks were found through LC-MS/MS. Ion pairs generated from eleven of these peptides (m/z?<?800) were quantified by MRM. Summed peak area ratios of 6 ion pairs from peptide m/z 520.3 (176.1, 353.7, 459.8, 503.3, 351.3, 593.1), which was identified as dihydroxyacetone kinase (DAK) fragment, decreased from mild to advanced stages of fibrosis or inflammation. Area Under Receiver Operating Characteristic Curves (AUROCs) of five ion models discriminating fibrosis degrees were 0.871?~?0.915 (S2-4 versus S0-1) and 0.804?~?0.924 (S3-4 versus S0-2). AUROCs discriminating inflammation grades were 0.840?~?0.902 (G2-4 versus G0-1) and 0.787?~?0.888 (G3-4 versus G0-2). The diagnostic power of these models provides improved sensitivity and specificity for predicting disease progression as compared to aspartate aminotransferase to platelet ratio index (APRI), FIB-4, Forn?s index and serum DAK protein.   Conclusions: The peptide fragment (m/z 520.3) of DAK is a promising biomarker to guide timing of antiviral treatment and to avoid liver biopsy in compensated CHB patients.
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/20744/
AV  - public
Y1  - 2013///
CY  - London
SN  - 1479-5876
A1  - Xu, Ming-Yi
A1  - Jia, Xiao-Fang
A1  - Qu, Ying
A1  - Zheng, Rui-Dan
A1  - Yuan, Zheng-Hong
A1  - Weng, Hong-Lei
A1  - Dooley, Steven
A1  - Wang, Xing-Peng
A1  - Zhang, Li-Jun
A1  - Lu, Lun-Gen
SP  - 1
JF  - Journal of Translational Medicine
IS  - 234
EP  - 14
VL  - 11
ER  -