eprintid: 20819
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/02/08/19
datestamp: 2016-06-09 13:17:04
lastmod: 2024-03-11 05:50:10
status_changed: 2016-06-09 13:17:04
type: article
metadata_visibility: show
creators_name: Dapunt, Ulrike
creators_name: Giese, Thomas
creators_name: Stegmaier, Sabine
creators_name: Moghaddam, Arash
creators_name: Hänsch, Gertrud Maria
title: The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms
subjects: ddc-610
divisions: i-911460
divisions: i-911600
abstract: Background: Implant infections are a major complication in the field of orthopaedics. Bacteria attach to the implant-surface and form biofilm-colonies which makes them difficult to treat. Not only immune cells exclusively respond to bacterial challenges, but also local tissue cells are capable of participating in defense mechanisms. The aim of this study was to evaluate the role of osteoblasts in the context of implant infections.   Methods: Primary osteoblasts were cultivated and stimulated with free-swimming bacteria at 4°C and 37°C. Supernatants were harvested for ELISA and expression of pro-inflammatory cytokines evaluated by RT-PCR. Bacterial binding to osteoblasts was evaluated using cytofluorometry and uptake was investigated by 3H thymidine-labelling of bacteria. Osteoblasts were additionally stimulated with the extracellular polymeric substance (EPS) of Staphylococcus epidermidis biofilms, as well as components of the EPS; the bacterial heat shock protein GroEL in particular.   Results: We demonstrated that binding of bacteria to the osteoblast cell surface leads to an increased production of pro-inflammatory cytokines. Bacteria are capable of surviving intracellular. Furthermore, osteoblasts do not only respond to free-swimming, planktonic bacteria, but also to components of the EPS, including lipoteichoic acid and the heat shock protein GroEL.   Conclusion: In conclusion, local tissue cells, specifically osteoblasts, might contribute to the persistence of the inflammatory response associated with implant-infections.
date: 2016
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1657503798
own_urn: urn:nbn:de:bsz:16-heidok-208193
language: eng
bibsort: DAPUNTULRITHEOSTEOBL2016
full_text_status: public
publication: BMC Musculoskeletal Disorders
volume: 17
number: 243
place_of_pub: London
pagerange: 1-9
issn: 1471-2474
citation:   Dapunt, Ulrike ; Giese, Thomas ; Stegmaier, Sabine ; Moghaddam, Arash ; Hänsch, Gertrud Maria  (2016) The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms.  BMC Musculoskeletal Disorders, 17 (243).  pp. 1-9.  ISSN 1471-2474     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/20819/1/12891_2016_Article_1091.pdf