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primary topic: Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment

Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment
type: Article
abstract: The present study was conducted as part of the research project "Tox-Box: securing drops of life - an enhanced health-related approach for risk assessment of drinking water in Germany”, funded by the Federal Ministry of Education and Research (02WRS1282G). The main fields of research within this project were endocrine disruption, genotoxicity, germ cell toxicity and neurotoxicity. The central task was to provide a compilation of methods to gain information about emerging pollutants to rank these in the health-related indicator value (HRIV) supported by the Federal Environmental Agency. The thesis, embedded in the field of neurotoxicity, aims at developing and validating a test battery for neurotoxicity, based on zebrafish embryo within scope of the 3R principle. The test systems comprised: (1) an extended embryo toxicity test (FET) to assess the general toxicity of substances and sediment extracts as well as to discriminate between neurotoxic and general physiological effects, (2) the acetylcholinesterase activity, (3) the lateral line system, (4) the olfactory epithelium and (5) the retina of zebrafish embryos. The methods were evaluated by screening test with various substances, namely amidotrizoic acid, caffeine, cypermethrin, 2,4-dichlorophenol, 2,4-dinitrotoluene, dichlorvos, 4-nonylphenol, paraoxon-methyl, perfluorooctanoic acid and perfluorooctane sulfonic acid. The extended embryo toxicity test proved useful to detect physiopathological effects and led to the determination of the EC10 value, which was used in subsequent neurotoxicity test to exclude side effects by physiological influences. The acetylcholinesterase test detects both, inhibition (dichlorvos / paraoxon) and activation (cypermethrin) and can be used as an in vivo and in vitro assay, as well as for pure substances and sediment extracts. A few substances were positive in one method exclusively, either in vivo or in vitro. Three out of nine substances triggered the test system at all, but, far bel…
date: 2016
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment (PDF)
has document: Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment (Other)
subject: 570 Biowissenschaften, Biologie

Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment (PDF)
type: Document
has file: Dissertation_Stengel_2016.pdf
has part: Dissertation_Stengel_2016.pdf

Daniel Stengel
type: Person
given name: Daniel
family name: Stengel

Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment (Other)
type: Document
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Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment (Other)
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Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment (Other)
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Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment (Other)
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Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment (Other)
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primary topic: Development and validation of a neurotoxicological test battery for neurotoxicity risk assessment

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en