title: miRNAs and sports: tracking training status and potentially confounding diagnoses
creator: Hecksteden, Anne
creator: Leidinger, Petra
creator: Backes, Christina
creator: Rheinheimer, Stefanie
creator: Pfeiffer, Mark
creator: Ferrauti, Alexander
creator: Kellmann, Michael
creator: Sedaghat, Farbod
creator: Meder, Benjamin
creator: Meese, Eckart
creator: Meyer, Tim
creator: Keller, Andreas
subject: ddc-610
subject: 610 Medical sciences Medicine
subject: ddc-796
subject: 796 Athletic and outdoor sports and games
description: Background: The dependency of miRNA abundance from physiological processes such as exercises remains partially understood. We set out to analyze the effect of physical exercises on miRNA profiles in blood and plasma of endurance and strength athletes in a systematic manner and correlated differentially abundant miRNAs in athletes to disease miRNAs biomarkers towards a better understanding of how physical exercise may confound disease diagnosis by miRNAs.   Methods: We profiled blood and plasma of 29 athletes before and after exercise. With four samples analyzed for each individual we analyzed 116 full miRNomes. The study set-up enabled paired analyses of individuals. Affected miRNAs were investigated for known disease associations using network analysis.   Results: MiRNA patterns in blood and plasma of endurance and strength athletes vary significantly with differences in blood outreaching variations in plasma. We found only moderate differences between the miRNA levels before training and the RNA levels after training as compared to the more obvious variations found between strength athletes and endurance athletes. We observed significant variations in the abundance of miR-140-3p that is a known circulating disease markers (raw and adjusted p value of 5 × 10−12 and 4 × 10−7). Similarly, the levels of miR-140-5p and miR-650, both of which have been reported as makers for a wide range of human pathologies significantly depend on the training mode. Among the most affected disease categories we found acute myocardial infarction. MiRNAs, which are up-regulated in endurance athletes inhibit VEGFA as shown by systems biology analysis of experimentally validated target genes.   Conclusion: We provide evidence that the mode and the extent of training are important confounding factors for a miRNA based disease diagnosis.
publisher: BioMed Central
date: 2016
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserver/21637/1/12967_2016_Article_974.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-216376
identifier:   Hecksteden, Anne ; Leidinger, Petra ; Backes, Christina ; Rheinheimer, Stefanie ; Pfeiffer, Mark ; Ferrauti, Alexander ; Kellmann, Michael ; Sedaghat, Farbod ; Meder, Benjamin ; Meese, Eckart ; Meyer, Tim ; Keller, Andreas  (2016) miRNAs and sports: tracking training status and potentially confounding diagnoses.  Journal of Translational Medicine, 14 (219).  pp. 1-13.  ISSN 1479-5876     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/21637/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng