eprintid: 21749
rev_number: 11
eprint_status: archive
userid: 1589
dir: disk0/00/02/17/49
datestamp: 2016-08-18 12:25:22
lastmod: 2024-03-29 23:38:17
status_changed: 2016-08-18 12:25:22
type: article
metadata_visibility: show
creators_name: Mikulski, Zbigniew
creators_name: Hartmann, Petra
creators_name: Jositsch, Gitte
creators_name: Zasłona, Zbigniew
creators_name: Lips, Katrin S.
creators_name: Pfeil, Uwe
creators_name: Kurzen, Hjalmar
creators_name: Lohmeyer, Jürgen
creators_name: Clauss, Wolfgang G.
creators_name: Grau, Veronika
creators_name: Fronius, Martin
creators_name: Kummer, Wolfgang
title: Nicotinic receptors on rat alveolar macrophages dampen ATP-induced increase in cytosolic calcium concentration
subjects: ddc-610
divisions: i-61900
abstract: Background: Nicotinic acetylcholine receptors (nAChR) have been identified on a variety of cells of the immune system and are generally considered to trigger anti-inflammatory events. In the present study, we determine the nAChR inventory of rat alveolar macrophages (AM), and investigate the cellular events evoked by stimulation with nicotine.   Methods: Rat AM were isolated freshly by bronchoalveolar lavage. The expression of nAChR subunits was analyzed by RT-PCR, immunohistochemistry, and Western blotting. To evaluate function of nAChR subunits, electrophysiological recordings and measurements of intracellular calcium concentration ([Ca2+]i) were conducted.   Results: Positive RT-PCR results were obtained for nAChR subunits α3, α5, α9, α10, β1, and β2, with most stable expression being noted for subunits α9, α10, β1, and β2. Notably, mRNA coding for subunit α7 which is proposed to convey the nicotinic anti-inflammatory response of macrophages from other sources than the lung was not detected. RT-PCR data were supported by immunohistochemistry on AM isolated by lavage, as well as in lung tissue sections and by Western blotting. Neither whole-cell patch clamp recordings nor measurements of [Ca2+]i revealed changes in membrane current in response to ACh and in [Ca2+]i in response to nicotine, respectively. However, nicotine (100 μM), given 2 min prior to ATP, significantly reduced the ATP-induced rise in [Ca2+]i by 30%. This effect was blocked by α-bungarotoxin and did not depend on the presence of extracellular calcium.   Conclusions: Rat AM are equipped with modulatory nAChR with properties distinct from ionotropic nAChR mediating synaptic transmission in the nervous system. Their stimulation with nicotine dampens ATP-induced Ca2+-release from intracellular stores. Thus, the present study identifies the first acute receptor-mediated nicotinic effect on AM with anti-inflammatory potential.
date: 2010
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1658529359
own_urn: urn:nbn:de:bsz:16-heidok-217498
language: eng
bibsort: MIKULSKIZBNICOTINICR2010
full_text_status: public
publication: Respiratory Research
volume: 11
number: 133
place_of_pub: London
pagerange: 1-16
issn: 1465-993X
citation:   Mikulski, Zbigniew ; Hartmann, Petra ; Jositsch, Gitte ; Zasłona, Zbigniew ; Lips, Katrin S. ; Pfeil, Uwe ; Kurzen, Hjalmar ; Lohmeyer, Jürgen ; Clauss, Wolfgang G. ; Grau, Veronika ; Fronius, Martin ; Kummer, Wolfgang  (2010) Nicotinic receptors on rat alveolar macrophages dampen ATP-induced increase in cytosolic calcium concentration.  Respiratory Research, 11 (133).  pp. 1-16.  ISSN 1465-993X     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/21749/1/12931_2010_Article_976.pdf