eprintid: 21844
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/02/18/44
datestamp: 2016-09-20 13:01:14
lastmod: 2024-03-09 18:51:19
status_changed: 2016-09-20 13:01:14
type: article
metadata_visibility: show
creators_name: Huang, Li-Jun
creators_name: Li, Ning
creators_name: Thurow, Corinna
creators_name: Wirtz, Markus
creators_name: Hell, Rüdiger
creators_name: Gatz, Christiane
title: Ectopically expressed glutaredoxin ROXY19 negatively regulates the detoxification pathway in Arabidopsis thaliana
subjects: ddc-580
divisions: i-718000
divisions: i-721000
abstract: Background: Glutaredoxins (GRXs) are small proteins which bind glutathione to either reduce disulfide bonds or to coordinate iron sulfur clusters. Whereas these well-established functions are associated with ubiquitously occurring GRXs that encode variants of a CPYC or a CGFS motif in the active center, land plants also possess CCxC/S-type GRXs (named ROXYs) for which the biochemical functions are yet unknown. ROXYs physically and genetically interact with bZIP transcription factors of the TGA family. In Arabidopsis, ectopically expressed ROXY19 (originally named GRX480 or GRXC9) negatively regulates expression of jasmonic acid/ethylene-induced defense genes through an unknown mechanism that requires at least one of the redundant transcription factors TGA2, TGA5 or TGA6.   Results: Ectopically expressed ROXY19 interferes with the activation of TGA-dependent detoxification genes. Similar to the tga2 tga5 tga6 mutant, 35S:ROXY19 plants are more susceptible to the harmful chemical TIBA (2,3,5-triiodobenzoic acid). The repressive function of ROXY19 depends on the integrity of the active site, which can be either CCMC or CPYC but not SSMS. Ectopic expression of the related GRX ROXY18/GRXS13 also led to increased susceptibility to TIBA, indicating potential functional redundancy of members of the ROXY gene family. This redundancy might explain why roxy19 knock-out plants did not show a phenotype with respect to the regulation of the TIBA-induced detoxification program. Complementation of the tga2 tga5 tga6 mutant with either TGA5 or TGA5C186S, in which the single potential target-site of ROXY19 had been eliminated, did not reveal any evidence for a critical redox modification that might be important for controlling the detoxification program.   Conclusions: ROXY19 and related proteins of the ROXY gene family can function as negative regulators of TGA-dependent promoters controlling detoxification genes.
date: 2016
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1659120918
own_urn: urn:nbn:de:bsz:16-heidok-218445
language: eng
bibsort: HUANGLIJUNECTOPICALL2016
full_text_status: public
publication: BMC Plant Biology
volume: 16
number: 200
place_of_pub: London
pagerange: 1-12
issn: 1471-2229
citation:   Huang, Li-Jun ; Li, Ning ; Thurow, Corinna ; Wirtz, Markus ; Hell, Rüdiger ; Gatz, Christiane  (2016) Ectopically expressed glutaredoxin ROXY19 negatively regulates the detoxification pathway in Arabidopsis thaliana.  BMC Plant Biology, 16 (200).  pp. 1-12.  ISSN 1471-2229     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/21844/1/12870_2016_Article_886.pdf