eprintid: 21998
rev_number: 13
eprint_status: archive
userid: 1589
dir: disk0/00/02/19/98
datestamp: 2016-10-26 12:42:10
lastmod: 2024-03-29 22:11:26
status_changed: 2016-10-26 12:42:10
type: article
metadata_visibility: show
creators_name: Günther, Frank
creators_name: Merle, Uta
creators_name: Frank, Uwe
creators_name: Gaida, Matthias M.
creators_name: Mutters, Nico T.
title: Pseudobacteremia outbreak of biofilm-forming Achromobacter xylosoxidans – environmental transmission
subjects: 610
divisions: 910100
divisions: 911700
divisions: 912000
abstract: Background:  Achromobacter xylosoxidans (AX) is known for intrinsic resistance to disinfectants. Our laboratory routine surveillance system detected an unexpected rise in AX bloodstream infections in a 2200-bed hospital. An epidemiological investigation was conducted to find the source and disrupt further transmission.   Methods: Outbreak cases were defined as patients with at least one positive blood culture positive for AX from May 2014 to May 2015. Medical records were reviewed, affected wards, as well as the microbiology laboratory were audited. Additionally, microbiologic culture and biofilm staining for suspected antiseptic reusable tissue dispensers were performed, and isolated AX strains were typed using RAPD PCR and PFGE.   Results: During the outbreak period, AX were isolated from blood cultures from 26 patients. The retrospective cohort study did not reveal common risk factors. The clinical features of the case patients suggested a pseudobacteremia. The reusable tissue dispensers containing Incidin® Plus solution product were found to be contaminated with biofilm-forming AX. Typing of the isolates revealed that blood culture isolates were identical with the strains found in the dispensers.   Conclusions: After changing the usage of the product to single-use and educating staff, the outbreak was terminated. Contamination of dispensers occurred due to insufficient reprocessing, since biofilm disrupting steps were not included in the process.
date: 2016
publisher: BioMed Central; Springer
id_scheme: DOI
ppn_swb: 1653447281
own_urn: urn:nbn:de:bsz:16-heidok-219980
language: eng
bibsort: GUNTHERFRAPSEUDOBACT2016
full_text_status: public
publication: BMC Infectious Diseases
volume: 16
number: 584
place_of_pub: London; Berlin; Heidelberg
pagerange: 1-5
issn: 1471-2334
citation:   Günther, Frank ; Merle, Uta ; Frank, Uwe ; Gaida, Matthias M. ; Mutters, Nico T.  (2016) Pseudobacteremia outbreak of biofilm-forming Achromobacter xylosoxidans – environmental transmission.  BMC Infectious Diseases, 16 (584).  pp. 1-5.  ISSN 1471-2334     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/21998/1/12879_2016_Article_1909.pdf