<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Massively parallelized STED nanoscopy"^^ . "Fluorescence microscopy constitutes a key method for the virtually\r\nnon-invasive study of biological structures and processes on a subcellular\r\nscale. Stimulated Emission Depletion (STED) nanoscopy extends\r\nfluorescence imaging to nanometer resolutions. However, this\r\nmethod usually acquires an image by scanning small pixels in a sequential\r\nmanner, which can lead to long acquisition times of several\r\nminutes. This limits the feasibility of many large-scale superresolution\r\nexperiments, because faster acquisition times imply sacrificing\r\neither highest resolution, a good signal-to-noise ratio or a large image\r\nsize. To unleash the full spatio-temporal resolving power potential of\r\nSTED nanoscopy on a large imaging region, massively parallelized\r\nacquisition is therefore inevitable.\r\nIn this thesis, I develop a comprehensive and quantitative description\r\nof parallelized STED and validate the derived findings by means\r\nof two experimental implementations. Investigating the key technical\r\nparameters and their interplay reveals the possibility of reducing the\r\nlaser power by up to three orders of magnitude below the value required\r\nfor serially acquiring STED systems. This eliminates a major\r\nbottleneck of previously reported attempts to parallelize STED nanoscopy,\r\nlimiting them to tiny image sizes or inferior resolutions. Moreover,\r\nusing a rather simple optical arrangement, I was able to enlarge\r\nthe superresolved image area to 33 μm edge length (roughly the dimensions\r\nof a small cell), featuring a resolution down to 30 nm and a\r\n13000-fold degree of parallelization. While the implementations presented\r\nhere should be viewed as prototypes, they prove the technical\r\nfeasibility of massively parallelized STED nanoscopy. The methods\r\ndeveloped in this thesis in combination with suitable high-speed detectors\r\nbring video-rate STED nanoscopy of whole cells within reach."^^ . "2016" . . . . . . . "Fabian Manuel"^^ . "Bergermann"^^ . "Fabian Manuel Bergermann"^^ . . . . . . "Massively parallelized STED nanoscopy (PDF)"^^ . . . "Dissertation_FabianBergermann.pdf"^^ . . . "Massively parallelized STED nanoscopy (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Massively parallelized STED nanoscopy (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Massively parallelized STED nanoscopy (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Massively parallelized STED nanoscopy (Other)"^^ . . . . . . "small.jpg"^^ . . . "Massively parallelized STED nanoscopy (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #22323 \n\nMassively parallelized STED nanoscopy\n\n" . "text/html" . . . "530 Physik"@de . "530 Physics"@en . . . "600 Technik, Medizin, angewandte Wissenschaften"@de . "600 Technology (Applied sciences)"@en . .