title: Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells
creator: Wachsmuth, Malte
creator: Knoch, Tobias A.
creator: Rippe, Karsten
subject: 610
subject: 610 Medical sciences Medicine
description: Background: Genome organization into subchromosomal topologically associating domains (TADs) is linked to cell-type-specific gene expression programs. However, dynamic properties of such domains remain elusive, and it is unclear how domain plasticity modulates genomic accessibility for soluble factors.   Results: Here, we combine and compare a high-resolution topology analysis of interacting chromatin loci with fluorescence correlation spectroscopy measurements of domain dynamics in single living cells. We identify topologically and dynamically independent chromatin domains of ~1 Mb in size that are best described by a loop-cluster polymer model. Hydrodynamic relaxation times and gyration radii of domains are larger for open (161 ± 15 ms, 297 ± 9 nm) than for dense chromatin (88 ± 7 ms, 243 ± 6 nm) and increase globally upon chromatin hyperacetylation or ATP depletion.   Conclusions: Based on the domain structure and dynamics measurements, we propose a loop-cluster model for chromatin domains. It suggests that the regulation of chromatin accessibility for soluble factors displays a significantly stronger dependence on factor concentration than search processes within a static network.
publisher: BioMed Central
date: 2016
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/22431/1/13072_2016_Article_93.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-224311
identifier:   Wachsmuth, Malte ; Knoch, Tobias A. ; Rippe, Karsten  (2016) Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells.  Epigenetics & Chromatin, 9 (57).  pp. 1-20.  ISSN 1756-8935     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/22431/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng