%0 Journal Article
%@ 1756-8935
%A Wachsmuth, Malte
%A Knoch, Tobias A.
%A Rippe, Karsten
%C London
%D 2016
%F heidok:22431
%I BioMed Central
%J Epigenetics & Chromatin
%N 57
%P 1-20
%T Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells
%U https://archiv.ub.uni-heidelberg.de/volltextserver/22431/
%V 9
%X Background: Genome organization into subchromosomal topologically associating domains (TADs) is linked to cell-type-specific gene expression programs. However, dynamic properties of such domains remain elusive, and it is unclear how domain plasticity modulates genomic accessibility for soluble factors.   Results: Here, we combine and compare a high-resolution topology analysis of interacting chromatin loci with fluorescence correlation spectroscopy measurements of domain dynamics in single living cells. We identify topologically and dynamically independent chromatin domains of ~1 Mb in size that are best described by a loop-cluster polymer model. Hydrodynamic relaxation times and gyration radii of domains are larger for open (161 ± 15 ms, 297 ± 9 nm) than for dense chromatin (88 ± 7 ms, 243 ± 6 nm) and increase globally upon chromatin hyperacetylation or ATP depletion.   Conclusions: Based on the domain structure and dynamics measurements, we propose a loop-cluster model for chromatin domains. It suggests that the regulation of chromatin accessibility for soluble factors displays a significantly stronger dependence on factor concentration than search processes within a static network.