TY  - JOUR
PB  - BioMed Central
A1  - Wachsmuth, Malte
A1  - Knoch, Tobias A.
A1  - Rippe, Karsten
TI  - Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells
IS  - 57
SP  - 1
AV  - public
N2  - Background: Genome organization into subchromosomal topologically associating domains (TADs) is linked to cell-type-specific gene expression programs. However, dynamic properties of such domains remain elusive, and it is unclear how domain plasticity modulates genomic accessibility for soluble factors.   Results: Here, we combine and compare a high-resolution topology analysis of interacting chromatin loci with fluorescence correlation spectroscopy measurements of domain dynamics in single living cells. We identify topologically and dynamically independent chromatin domains of ~1 Mb in size that are best described by a loop-cluster polymer model. Hydrodynamic relaxation times and gyration radii of domains are larger for open (161 ± 15 ms, 297 ± 9 nm) than for dense chromatin (88 ± 7 ms, 243 ± 6 nm) and increase globally upon chromatin hyperacetylation or ATP depletion.   Conclusions: Based on the domain structure and dynamics measurements, we propose a loop-cluster model for chromatin domains. It suggests that the regulation of chromatin accessibility for soluble factors displays a significantly stronger dependence on factor concentration than search processes within a static network.
CY  - London
VL  - 9
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/22431/
ID  - heidok22431
SN  - 1756-8935
JF  - Epigenetics & Chromatin
EP  - 20
Y1  - 2016///
ER  -