eprintid: 22431
rev_number: 14
eprint_status: archive
userid: 1589
dir: disk0/00/02/24/31
datestamp: 2017-01-04 13:23:00
lastmod: 2024-03-09 07:10:16
status_changed: 2017-01-04 13:23:00
type: article
metadata_visibility: show
creators_name: Wachsmuth, Malte
creators_name: Knoch, Tobias A.
creators_name: Rippe, Karsten
title: Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells
subjects: 610
divisions: 716000
divisions: 718000
divisions: 850300
divisions: 850800
abstract: Background: Genome organization into subchromosomal topologically associating domains (TADs) is linked to cell-type-specific gene expression programs. However, dynamic properties of such domains remain elusive, and it is unclear how domain plasticity modulates genomic accessibility for soluble factors.   Results: Here, we combine and compare a high-resolution topology analysis of interacting chromatin loci with fluorescence correlation spectroscopy measurements of domain dynamics in single living cells. We identify topologically and dynamically independent chromatin domains of ~1 Mb in size that are best described by a loop-cluster polymer model. Hydrodynamic relaxation times and gyration radii of domains are larger for open (161 ± 15 ms, 297 ± 9 nm) than for dense chromatin (88 ± 7 ms, 243 ± 6 nm) and increase globally upon chromatin hyperacetylation or ATP depletion.   Conclusions: Based on the domain structure and dynamics measurements, we propose a loop-cluster model for chromatin domains. It suggests that the regulation of chromatin accessibility for soluble factors displays a significantly stronger dependence on factor concentration than search processes within a static network.
date: 2016
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1655361724
own_urn: urn:nbn:de:bsz:16-heidok-224311
language: eng
bibsort: WACHSMUTHMDYNAMICPRO2016
full_text_status: public
publication: Epigenetics & Chromatin
volume: 9
number: 57
place_of_pub: London
pagerange: 1-20
issn: 1756-8935
citation:   Wachsmuth, Malte ; Knoch, Tobias A. ; Rippe, Karsten  (2016) Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells.  Epigenetics & Chromatin, 9 (57).  pp. 1-20.  ISSN 1756-8935     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/22431/1/13072_2016_Article_93.pdf