eprintid: 22432
rev_number: 13
eprint_status: archive
userid: 1589
dir: disk0/00/02/24/32
datestamp: 2017-01-04 13:29:50
lastmod: 2024-05-08 04:43:23
status_changed: 2017-01-04 13:29:50
type: article
metadata_visibility: show
creators_name: Knoch, Tobias A.
creators_name: Wachsmuth, Malte
creators_name: Kepper, Nick
creators_name: Lesnussa, Michael
creators_name: Abuseiris, Anis
creators_name: Ali Imam, A. M.
creators_name: Kolovos, Petros
creators_name: Zuin, Jessica
creators_name: Kockx, Christel E. M.
creators_name: Brouwer, Rutger W. W.
creators_name: van de Werken, Harmen J. G.
creators_name: van IJcken, Wilfred F. J.
creators_name: Wendt, Kerstin S.
creators_name: Grosveld, Frank G.
title: The detailed 3D multi-loop aggregate/rosette chromatin architecture and functional dynamic organization of the human and mouse genomes
subjects: ddc-610
divisions: i-716000
divisions: i-850300
divisions: i-850800
abstract: Background: The dynamic three-dimensional chromatin architecture of genomes and its co-evolutionary connection to its function—the storage, expression, and replication of genetic information—is still one of the central issues in biology. Here, we describe the much debated 3D architecture of the human and mouse genomes from the nucleosomal to the megabase pair level by a novel approach combining selective high-throughput high-resolution chromosomal interaction capture (T2C), polymer simulations, and scaling analysis of the 3D architecture and the DNA sequence.   Results: The genome is compacted into a chromatin quasi-fibre with ~5 ± 1 nucleosomes/11 nm, folded into stable ~30–100 kbp loops forming stable loop aggregates/rosettes connected by similar sized linkers. Minor but significant variations in the architecture are seen between cell types and functional states. The architecture and the DNA sequence show very similar fine-structured multi-scaling behaviour confirming their co-evolution and the above.   Conclusions: This architecture, its dynamics, and accessibility, balance stability and flexibility ensuring genome integrity and variation enabling gene expression/regulation by self-organization of (in)active units already in proximity. Our results agree with the heuristics of the field and allow “architectural sequencing” at a genome mechanics level to understand the inseparable systems genomic properties.
date: 2016
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1655358073
own_urn: urn:nbn:de:bsz:16-heidok-224325
language: eng
bibsort: KNOCHTOBIATHEDETAILE2016
full_text_status: public
publication: Epigenetics & Chromatin
volume: 9
number: 58
place_of_pub: London
pagerange: 1-22
issn: 1756-8935
citation:   Knoch, Tobias A. ; Wachsmuth, Malte ; Kepper, Nick ; Lesnussa, Michael ; Abuseiris, Anis ; Ali Imam, A. M. ; Kolovos, Petros ; Zuin, Jessica ; Kockx, Christel E. M. ; Brouwer, Rutger W. W. ; van de Werken, Harmen J. G. ; van IJcken, Wilfred F. J. ; Wendt, Kerstin S. ; Grosveld, Frank G.  (2016) The detailed 3D multi-loop aggregate/rosette chromatin architecture and functional dynamic organization of the human and mouse genomes.  Epigenetics & Chromatin, 9 (58).  pp. 1-22.  ISSN 1756-8935     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/22432/1/13072_2016_Article_89.pdf