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primary topic: Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury

Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury
type: Article
abstract: Spinal cord injury (SCI) is characterized by axonal damage, neural degeneration, formation of cystic cavities, and upregulation of a plethora of inhibitory as well as inflammatory molecules. To protect the surrounding tissue from further damage, fibroblasts and reactive astrocytes form an impenetrable barrier lining the lesion site. This environment impedes endogenous regeneration of axotomized neurons and glial cells. In addition to being exposed to extrinsic inhibitors of axonal regeneration after SCI, neurons in the mammalian central nervous system (CNS) intrinsically lack the capacity for spontaneous axonal regeneration. To restore neural tissue integrity and provide a favorable environment for injured axons to regenerate, an appropriate substrate is needed. An optimal substrate for neuroregeneration should have a neural identity and fulfill several functions including physical support and guidance, trophic and metabolic support, maintenance of tissue homeostasis, and modulation of neuronal outgrowth and network activity. Astrocytes represent the most suitable cell population to fulfill this task. Induced pluripotent stem cells (iPSCs), which show many homologies to embryonic stem cells (ESCs), represent an ethically acceptable means to obtain large amounts of astrocytes in vitro for autologous transplantation. In the present study, populations of immature astrocytes with caudal identity were generated from three human pluripotent stem cell (PSC) lines, whereby terminal in vitro differentiation was performed according to previously published studies using the inductors of astrocytic maturation CNTF, BMP2/4 and FGF1 to allow for comparative analysis of specific astrocytic subtypes and for selection of a pro-regenerative cell population. In accordance with previously published findings, morphological and functional differences among astrocytic populations were observed in the present study. Compared to astrocytes differentiated with FBS only, astrocytes differenti…
date: 2017
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (PDF)
has document: Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (Other)
subject: 570 Biowissenschaften, Biologie

Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (PDF)
type: Document
has file: Ina_Thesis_Final_File.pdf
has part: Ina_Thesis_Final_File.pdf

Ina Krasimirova Simeonova
type: Person
given name: Ina Krasimirova
family name: Simeonova

Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (Other)
type: Document
is version of: Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (PDF)
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Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (Other)
type: Document
is version of: Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (PDF)
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Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (Other)
type: Document
is version of: Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (PDF)
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Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (Other)
type: Document
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Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (Other)
type: Document
is version of: Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (PDF)
is volatile version of: Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury (PDF)
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HTML Summary of #22495 Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury
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primary topic: Generation of Defined Astrocytic Phenotypes from Human Pluripotent Stem Cells for Transplantation after Spinal Cord Injury

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en