title: Bacopa monnieri extract increases rat coronary flow and protects against myocardial ischemia/reperfusion injury
creator: Srimachai, Sirintorn
creator: Devaux, Sylvie
creator: Demougeot, Celine
creator: Kumphune, Sarawut
creator: Ullrich, Nina D.
creator: Niggli, Ernst
creator: Ingkaninan, Kornkanok
creator: Kamkaew, Natakorn
creator: Scholfield, C. Norman
creator: Tapechum, Sompol
creator: Chootip, Krongkarn
subject: ddc-610
subject: 610 Medical sciences Medicine
description: Background: This study explored Bacopa monnieri, a medicinal Ayurvedic herb, as a cardioprotectant against ischemia/reperfusion injury using cardiac function and coronary flow as end-points.   Methods: In normal isolated rat hearts, coronary flow, left ventricular developed pressure, heart rate, and functional recovery were measured using the Langendorff preparation. Hearts were perfused with either (i) Krebs-Henseleit (normal) solution, (control), or with 30, 100 μg/ml B. monnieri ethanolic extract (30 min), or (ii) with normal solution or extract for 10 min preceding no-perfusion ischemia (30 min) followed by reperfusion (30 min) with normal solution. Infarct volumes were measured by triphenyltetrazolium staining. L-type Ca2+-currents (ICa, L) were measured by whole-cell patching in HL-1 cells, a mouse atrial cardiomyocyte cell line. Cytotoxicity of B. monnieri was assessed in rat isolated ventricular myocytes by trypan blue exclusion.   Results: In normally perfused hearts, B. monnieri increased coronary flow by 63 ± 13% (30 μg/ml) and 216 ± 21% (100 μg/ml), compared to control (5 ± 3%) (n = 8–10, p < 0.001). B. monnieri treatment preceding ischemia/reperfusion improved left ventricular developed pressure by 84 ± 10% (30 μg/ml), 82 ± 10% (100 μg/ml) and 52 ± 6% (control) compared to pre- ischemia/reperfusion. Similarly, functional recovery showed a sustained increase. Moreover, B. monnieri (100 μg/ml) reduced the percentage of infarct size from 51 ± 2% (control) to 25 ± 2% (n = 6-8, p < 0.0001). B. monnieri (100 μg/ml) reduced ICa, L by 63 ± 4% in HL-1 cells. Ventricular myocyte survival decreased at higher concentrations (50–1000 μg/ml) B. monnieri.   Conclusions:  B. monnieri improves myocardial function following ischemia/reperfusion injury through recovery of coronary blood flow, contractile force and decrease in infarct size. Thus this may lead to a novel cardioprotectant strategy.
publisher: BioMed Central
date: 2017
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserver/22691/1/12906_2017_Article_1637.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-226910
identifier:   Srimachai, Sirintorn ; Devaux, Sylvie ; Demougeot, Celine ; Kumphune, Sarawut ; Ullrich, Nina D. ; Niggli, Ernst ; Ingkaninan, Kornkanok ; Kamkaew, Natakorn ; Scholfield, C. Norman ; Tapechum, Sompol ; Chootip, Krongkarn  (2017) Bacopa monnieri extract increases rat coronary flow and protects against myocardial ischemia/reperfusion injury.  BMC Complementary and Alternative Medicine, 17 (117).  pp. 1-10.  ISSN 1472-6882     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/22691/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng