TY  - JOUR
CY  - London
SN  - 1472-6882
TI  - Bacopa monnieri extract increases rat coronary flow and protects against myocardial ischemia/reperfusion injury
N2  - Background: This study explored Bacopa monnieri, a medicinal Ayurvedic herb, as a cardioprotectant against ischemia/reperfusion injury using cardiac function and coronary flow as end-points.   Methods: In normal isolated rat hearts, coronary flow, left ventricular developed pressure, heart rate, and functional recovery were measured using the Langendorff preparation. Hearts were perfused with either (i) Krebs-Henseleit (normal) solution, (control), or with 30, 100 ?g/ml B. monnieri ethanolic extract (30 min), or (ii) with normal solution or extract for 10 min preceding no-perfusion ischemia (30 min) followed by reperfusion (30 min) with normal solution. Infarct volumes were measured by triphenyltetrazolium staining. L-type Ca2+-currents (ICa, L) were measured by whole-cell patching in HL-1 cells, a mouse atrial cardiomyocyte cell line. Cytotoxicity of B. monnieri was assessed in rat isolated ventricular myocytes by trypan blue exclusion.   Results: In normally perfused hearts, B. monnieri increased coronary flow by 63?±?13% (30 ?g/ml) and 216?±?21% (100 ?g/ml), compared to control (5?±?3%) (n?=?8?10, p?<?0.001). B. monnieri treatment preceding ischemia/reperfusion improved left ventricular developed pressure by 84?±?10% (30 ?g/ml), 82?±?10% (100 ?g/ml) and 52?±?6% (control) compared to pre- ischemia/reperfusion. Similarly, functional recovery showed a sustained increase. Moreover, B. monnieri (100 ?g/ml) reduced the percentage of infarct size from 51?±?2% (control) to 25?±?2% (n?=?6-8, p?<?0.0001). B. monnieri (100 ?g/ml) reduced ICa, L by 63?±?4% in HL-1 cells. Ventricular myocyte survival decreased at higher concentrations (50?1000 ?g/ml) B. monnieri.   Conclusions:  B. monnieri improves myocardial function following ischemia/reperfusion injury through recovery of coronary blood flow, contractile force and decrease in infarct size. Thus this may lead to a novel cardioprotectant strategy.
ID  - heidok22691
PB  - BioMed Central
A1  - Srimachai, Sirintorn
A1  - Devaux, Sylvie
A1  - Demougeot, Celine
A1  - Kumphune, Sarawut
A1  - Ullrich, Nina D.
A1  - Niggli, Ernst
A1  - Ingkaninan, Kornkanok
A1  - Kamkaew, Natakorn
A1  - Scholfield, C. Norman
A1  - Tapechum, Sompol
A1  - Chootip, Krongkarn
JF  - BMC Complementary and Alternative Medicine
VL  - 17
AV  - public
EP  - 10
IS  - 117
SP  - 1
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/22691/
Y1  - 2017///
ER  -