<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Comparing bacterial gene networks based on high-throughput phenomics"^^ . "Novel genes are being discovered at constantly increasing rates by sequencing bacterial genomes and bacterial communities. Gene function discovery has been\r\nlagging behind, but recent technological advances allow us to apply reverse genetics approaches on a genome wide scale.\r\nIn this study I profile the growth of more than 3800 gene deletion mutants of the pathogen Salmonella Typhimurium in more than 550 perturbations including physical stresses, nutrient limitation, antibiotics and host defense molecules.\r\nAnalysis of gene-drug interaction scores reveal significant phenotypes for 75% of the tested mutants. The data set provides a number of novel biological inferences,\r\nlinking genes of unknown function to known pathways and providing insights into drug mode-of-action, uptake and efflux.\r\nUsing similar high-throughput data available for E. coli., I provide the first comprehensive cross-species comparison of genetic networks in bacteria. Correlation analysis and detection of functional modules reveals broad conservation of cellular pathways and drug responses between Salmonella and E. coli. However, I also find intriguing cases of network rewiring and investigate how species-specific genes connect to conserved modules.\r\nLastly, I investigate the highly different resistance levels of Salmonella and E. coli to the type 2 diabetes drug metformin and determine the Salmonella-specific\r\nefflux pump SmvA as the major component conferring drug resistance. Furthermore, I identify more transporters capable of exporting metformin and examine their wiring into the cellular networks of E. coli and Salmonella. This\r\nanalysis reveals that many enterobacteria may have the potential to develop resistance against metformin leading to important implications for diabetic patients."^^ . "2017" . . . . . . . "Birgit"^^ . "Pfalz"^^ . "Birgit Pfalz"^^ . . . . . . "Comparing bacterial gene networks based on high-throughput phenomics (PDF)"^^ . . . "Dissertation_BirgitPfalz.pdf"^^ . . . "Comparing bacterial gene networks based on high-throughput phenomics (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #22920 \n\nComparing bacterial gene networks based on high-throughput phenomics\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . .