<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper®"^^ . "Background: Accurate determination of the predictive markers human epidermal growth factor receptor 2 (HER2/ERBB2), estrogen receptor (ER/ESR1), progesterone receptor (PgR/PGR), and marker of proliferation Ki67 (MKI67) is indispensable for therapeutic decision making in early breast cancer. In this multicenter prospective study, we addressed the issue of inter- and intrasite reproducibility using the recently developed reverse transcription-quantitative real-time polymerase chain reaction-based MammaTyper® test. Methods: Ten international pathology institutions participated in this study and determined messenger RNA expression levels of ERBB2, ESR1, PGR, and MKI67 in both centrally and locally extracted RNA from formalin-fixed, paraffin-embedded breast cancer specimens with the MammaTyper® test. Samples were measured repeatedly on different days within the local laboratories, and reproducibility was assessed by means of variance component analysis, Fleiss’ kappa statistics, and interclass correlation coefficients (ICCs). Results: Total variations in measurements of centrally and locally prepared RNA extracts were comparable; therefore, statistical analyses were performed on the complete dataset. Intersite reproducibility showed total SDs between 0.21 and 0.44 for the quantitative single-marker assessments, resulting in ICC values of 0.980–0.998, demonstrating excellent agreement of quantitative measurements. Also, the reproducibility of binary single-marker results (positive/negative), as well as the molecular subtype agreement, was almost perfect with kappa values ranging from 0.90 to 1.00. Conclusions: On the basis of these data, the MammaTyper® has the potential to substantially improve the current standards of breast cancer diagnostics by providing a highly precise and reproducible quantitative assessment of the established breast cancer biomarkers and molecular subtypes in a decentralized workup."^^ . "2017" . . "19" . "55" . . "BioMed Central"^^ . . . "Breast Cancer Research"^^ . . . "1465542X" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Robert"^^ . "Stoehr"^^ . "Robert Stoehr"^^ . . "Thomas"^^ . "Keller"^^ . "Thomas Keller"^^ . . "Zsuzsanna"^^ . "Varga"^^ . "Zsuzsanna Varga"^^ . . "Heikki"^^ . "Joensuu"^^ . "Heikki Joensuu"^^ . . "Hong"^^ . "Bu"^^ . "Hong Bu"^^ . . "Arndt"^^ . "Hartmann"^^ . "Arndt Hartmann"^^ . . "Peter"^^ . "Schraml"^^ . "Peter Schraml"^^ . . "Claudia"^^ . "Gürtler"^^ . "Claudia Gürtler"^^ . . "Frederique"^^ . "Penault-Llorca"^^ . "Frederique Penault-Llorca"^^ . . "Kornelia"^^ . "Schlombs"^^ . "Kornelia Schlombs"^^ . . "Fraser"^^ . "Symmans"^^ . "Fraser Symmans"^^ . . "Elena"^^ . "Guerini-Rocco"^^ . "Elena Guerini-Rocco"^^ . . "Xiaodong"^^ . "Teng"^^ . "Xiaodong Teng"^^ . . "Giuseppe"^^ . "Viale"^^ . "Giuseppe Viale"^^ . . "Annette"^^ . "Lebeau"^^ . "Annette Lebeau"^^ . . "Ugur"^^ . "Sahin"^^ . "Ugur Sahin"^^ . . "Reinhard"^^ . "von Wasielewski"^^ . "Reinhard von Wasielewski"^^ . . "John"^^ . "Bartlett"^^ . "John Bartlett"^^ . . "Hans-Peter"^^ . "Sinn"^^ . "Hans-Peter Sinn"^^ . . "Andreas"^^ . "Turzynski"^^ . "Andreas Turzynski"^^ . . "Mark"^^ . "Laible"^^ . "Mark Laible"^^ . . . . . . "An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper® (PDF)"^^ . . . "13058_2017_Article_848.pdf"^^ . . . "An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper® (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper® (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper® (Other)"^^ . . . . . . "preview.jpg"^^ . . . "An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper® (Other)"^^ . . . . . . "medium.jpg"^^ . . . "An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper® (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #22945 \n\nAn international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper®\n\n" . "text/html" . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .