<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction"^^ . "Background: In this study we evaluated the interactions of human adipose tissue-derived stem cells (ADSCs) and different human breast cancer cell lines (BRCAs) with regard to the safety of cell-assisted lipotransfers for breast reconstruction and a thereby unintended co-localization of ADSCs and BRCAs. Methods: ADSCs were co-cultured with five different human BRCAs (MCF-7, MDA-MB-231, SK-BR-3, ZR-75-30, and EVSA-T) and primary BRCAs from one patient in a transwell system, and cell-cell-interactions were analyzed by assessing doubling time, migration and invasion, angiogenesis, quantitative real-time polymerase chain reaction (PCR) of more than 300 tumor-associated genes, and multiplex protein assays of 20 chemokines and growth factors and eight matrix metalloproteinases (MMPs). Results of co-culture were compared to those of the respective monoculture. Results: Quantitative real-time PCR revealed remarkable changes in the expression of multiple tumor-associated genes in co-culture compared to monocultures of both ADSCs and BRCAs. Concomitantly, the concentration of several tumor-associated proteins, such as cytokines and MMPs, were strongly increased in co-culture. Furthermore, exclusively in co-culture with ADSCs, the different BRCAs were exposed to several important tumor-modulating proteins, such as CCL2, HGF, or interleukins. Co-culture did not significantly affect cellular proliferation of either ADSCs or BRCAs (p > 0.05). The migration of MCF-7 and MDA-MB-231 BRCAs was significantly increased in co-culture with ADSCs by a mean of 11% and 23%, respectively (p = 0.04 and 0.012), as well as that of ADSCs in co-culture with MDA-MB-231, ZR-75-30, and EVSA-T (+11–15%, p = 0.035–0.045). Co-culture with MDA-MB-231, SK-BR-3, and EVSA-T BRCAs significantly increased the invasive behavior of ADSCs by a mean of 24–41% (p = 0.014–0.039). There were no significant differences in the in vitro invasive properties of BRCAs in co-culture compared to monoculture. An in vitro angiogenesis assay revealed an increased tube formation of conditioned media from co-cultured BRCAs and ADSCs compared to the respective monocultures. Conclusion: This study further elucidates the possible interactions of primary human ADSCs with human BRCAs, pointing towards a potential increased oncological risk which should not be neglected when considering a clinical use of cell-assisted lipoaspirates in breast reconstruction."^^ . "2017" . . "8" . "121" . . "BioMed Central"^^ . . . "Stem Cell Research & Therapy"^^ . . . "17576512" . . . . . . . . . . . . . . . . . . . . . . . . . . "Frederik"^^ . "Marmé"^^ . "Frederik Marmé"^^ . . "Stefanie"^^ . "Sandmann"^^ . "Stefanie Sandmann"^^ . . "Inka"^^ . "Zörnig"^^ . "Inka Zörnig"^^ . . "Günter"^^ . "Germann"^^ . "Günter Germann"^^ . . "Eva"^^ . "Köllensperger"^^ . "Eva Köllensperger"^^ . . "Lilly-Claire"^^ . "Bonnert"^^ . "Lilly-Claire Bonnert"^^ . . "Uwe"^^ . "Leimer"^^ . "Uwe Leimer"^^ . . "Felix"^^ . "Gramley"^^ . "Felix Gramley"^^ . . . . . . "The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction (PDF)"^^ . . . "13287_2017_Article_579.pdf"^^ . . . "The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction (Other)"^^ . . . . . . "preview.jpg"^^ . . . "The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction (Other)"^^ . . . . . . "medium.jpg"^^ . . . "The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction (Other)"^^ . . . . . . "small.jpg"^^ . . . "The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #22995 \n\nThe impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction\n\n" . "text/html" . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .