eprintid: 22995
rev_number: 11
eprint_status: archive
userid: 1589
dir: disk0/00/02/29/95
datestamp: 2017-05-29 09:52:34
lastmod: 2024-05-25 00:37:34
status_changed: 2017-05-29 09:52:34
type: article
metadata_visibility: show
creators_name: Köllensperger, Eva
creators_name: Bonnert, Lilly-Claire
creators_name: Zörnig, Inka
creators_name: Marmé, Frederik
creators_name: Sandmann, Stefanie
creators_name: Germann, Günter
creators_name: Gramley, Felix
creators_name: Leimer, Uwe
title: The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction
subjects: ddc-610
divisions: i-910100
abstract: Background: In this study we evaluated the interactions of human adipose tissue-derived stem cells (ADSCs) and different human breast cancer cell lines (BRCAs) with regard to the safety of cell-assisted lipotransfers for breast reconstruction and a thereby unintended co-localization of ADSCs and BRCAs.   Methods: ADSCs were co-cultured with five different human BRCAs (MCF-7, MDA-MB-231, SK-BR-3, ZR-75-30, and EVSA-T) and primary BRCAs from one patient in a transwell system, and cell-cell-interactions were analyzed by assessing doubling time, migration and invasion, angiogenesis, quantitative real-time polymerase chain reaction (PCR) of more than 300 tumor-associated genes, and multiplex protein assays of 20 chemokines and growth factors and eight matrix metalloproteinases (MMPs). Results of co-culture were compared to those of the respective monoculture.   Results: Quantitative real-time PCR revealed remarkable changes in the expression of multiple tumor-associated genes in co-culture compared to monocultures of both ADSCs and BRCAs. Concomitantly, the concentration of several tumor-associated proteins, such as cytokines and MMPs, were strongly increased in co-culture. Furthermore, exclusively in co-culture with ADSCs, the different BRCAs were exposed to several important tumor-modulating proteins, such as CCL2, HGF, or interleukins. Co-culture did not significantly affect cellular proliferation of either ADSCs or BRCAs (p > 0.05). The migration of MCF-7 and MDA-MB-231 BRCAs was significantly increased in co-culture with ADSCs by a mean of 11% and 23%, respectively (p = 0.04 and 0.012), as well as that of ADSCs in co-culture with MDA-MB-231, ZR-75-30, and EVSA-T (+11–15%, p = 0.035–0.045). Co-culture with MDA-MB-231, SK-BR-3, and EVSA-T BRCAs significantly increased the invasive behavior of ADSCs by a mean of 24–41% (p = 0.014–0.039). There were no significant differences in the in vitro invasive properties of BRCAs in co-culture compared to monoculture. An in vitro angiogenesis assay revealed an increased tube formation of conditioned media from co-cultured BRCAs and ADSCs compared to the respective monocultures.   Conclusion: This study further elucidates the possible interactions of primary human ADSCs with human BRCAs, pointing towards a potential increased oncological risk which should not be neglected when considering a clinical use of cell-assisted lipoaspirates in breast reconstruction.
date: 2017
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1659211514
own_urn: urn:nbn:de:bsz:16-heidok-229951
language: eng
bibsort: KOLLENSPERTHEIMPACTO2017
full_text_status: public
publication: Stem Cell Research & Therapy
volume: 8
number: 121
place_of_pub: London
pagerange: 1-19
issn: 1757-6512
citation:   Köllensperger, Eva ; Bonnert, Lilly-Claire ; Zörnig, Inka ; Marmé, Frederik ; Sandmann, Stefanie ; Germann, Günter ; Gramley, Felix ; Leimer, Uwe  (2017) The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction.  Stem Cell Research & Therapy, 8 (121).  pp. 1-19.  ISSN 1757-6512     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/22995/1/13287_2017_Article_579.pdf