eprintid: 23024
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/02/30/24
datestamp: 2017-06-13 12:49:16
lastmod: 2024-03-11 04:30:48
status_changed: 2017-06-13 12:49:16
type: article
metadata_visibility: show
creators_name: Ghassem‑Zadeh, Sahar
creators_name: Gaida, Matthias M.
creators_name: Szanyi, Szilard
creators_name: Acha-Orbea, Hans
creators_name: Frossard, Jean-Louis
creators_name: Hinz, Ulf
creators_name: Hackert, Thilo
creators_name: Strobel, Oliver
creators_name: Felix, Klaus
title: Distinct pathophysiological cytokine profiles for discrimination between autoimmune pancreatitis, chronic pancreatitis, and pancreatic ductal adenocarcinoma
subjects: ddc-610
divisions: i-910200
divisions: i-912000
abstract: Background: Discriminating between autoimmune pancreatitis (AIP), chronic pancreatitis (CP), and pancreatic ductal adenocarcinoma (PDAC) can be challenging. In this retrospective study, levels of serum and tissue cytokines were analyzed as part of the clinical strategy for the preoperative differentiation between AIP and PDAC. The identification of differential cytokine profiles may help to prevent unnecessary surgical resection and allow optimal treatment of these pathologies.   Methods: To compare the cytokine profiles of AIP, CP, and PDAC patients, serum and pancreatic tissue homogenates were subjected to multiplex analysis of 17 inflammatory mediators. In total, serum from 73 patients, composed of 29 AIP (14 AIP-1 and 15 AIP-2), 17 CP, and 27 PDAC, and pancreatic tissue from 36 patients, including 12 AIP (six AIP-1 and six AIP-2), 12 CP, and 12 PDAC, were analyzed.   Results: Comparing AIP and PDAC patients’ serum, significantly higher concentrations were found in AIP for interleukins IL-1β, IL-7, IL-13, and granulocyte colony-stimulating factor (G-CSF). G-CSF also allowed discrimination of AIP from CP. Furthermore, once AIP was divided into subtypes, significantly higher serum levels for IL-7 and G-CSF were measured in both subtypes of AIP and in AIP-2 for IL-1β when compared to PDAC. G-CSF and TNF-α were also significantly differentially expressed in tissue homogenates between AIP-2 and PDAC.   Conclusions: The cytokines IL-1β, IL-7, and G-CSF can be routinely measured in patients’ serum, providing an elegant and non-invasive approach for differential diagnosis. G-CSF is a good candidate to supplement the currently known serum markers in predictive tests for AIP and represents a basis for a combined blood test to differentiate AIP and particularly AIP-2 from PDAC, enhancing the possibility of appropriate treatment.
date: 2017
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1659253314
own_urn: urn:nbn:de:bsz:16-heidok-230243
language: eng
bibsort: GHASSEMZADDISTINCTPA2017
full_text_status: public
publication: Journal of Translational Medicine
volume: 15
number: 126
place_of_pub: London
pagerange: 1-11
issn: 1479-5876
citation:   Ghassem‑Zadeh, Sahar ; Gaida, Matthias M. ; Szanyi, Szilard ; Acha-Orbea, Hans ; Frossard, Jean-Louis ; Hinz, Ulf ; Hackert, Thilo ; Strobel, Oliver ; Felix, Klaus  (2017) Distinct pathophysiological cytokine profiles for discrimination between autoimmune pancreatitis, chronic pancreatitis, and pancreatic ductal adenocarcinoma.  Journal of Translational Medicine, 15 (126).  pp. 1-11.  ISSN 1479-5876     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/23024/1/12967_2017_Article_1227.pdf