%0 Generic
%A Frohnmayer, Johannes
%D 2018
%F heidok:23081
%R 10.11588/heidok.00023081
%T Bottom-Up Assembly of Synthetic Model  Systems for Cellular Adhesion
%U https://archiv.ub.uni-heidelberg.de/volltextserver/23081/
%X The second law of thermodynamics requires life processes to be separated by a  boundary from their environment. Therefore, all cells are encapsulated by a lipid  membrane. This enables life’s great degree of organization. To allow nutrients and  information to pass this barrier, cells use various transmembrane proteins for material  and signal transduction. When creating artificial cells, synthetic biology has  to account for these facts. This thesis presents the development of such a stable  and easy-to-manipulate protocell model for cellular adhesion experiments. First  adhesion of small unilamellar vesicles containing reconstituted blood-platelet integrin  to functionalized surfaces were studied using quartz crystal microbalance. Due  to restrictions of giant unilamellar vesicles (GUV), a novel compartment system,  named droplet-stabilized giant unilamellar vesicles (dsGUV), was created combining  the versatility of microfluidics, the stability of surfactant-stabilized water-in-oil  droplets, with the high level of bio-mimicry of lipid membranes. Through the use  of microfluidic pico-injection technology, components can be sequentially added to  the compartment, pathing the way for more complex and diverse model systems.  In this work, reconstitution of integrin into dsGUVs is presented. The system was  characterized using different methods. Finally the recovery of GUVs from dsGUVs  to a physiological environment is presented.