<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "siRNA SCREEN FOR IDENTIFICATION OF HUMAN KINASES INVOLVED IN ASSEMBLY AND RELEASE OF HIV-1"^^ . "The replication of the human immunodeficiency virus type 1 (HIV-1) is as yet not fully understood. In particular the knowledge of interactions between viral and host cell proteins and the understanding of complete virus-host protein networks are still imprecise. An integral picture of the hijacked cellular machinery is essential for a better comprehension of the virus. And as a prerequisite, new tools are needed for this purpose.\r\nTo create such a novel tool, a screening platform for host cell factors was established in this work. The screening assay serves as a powerful method to gain insights into virus-host-interactions. It was specifically tailored to addressing the stage of assembly and release of viral particles during the replication cycle of HIV-1. It was designed to be suitable for both RNAi and chemical compound screening. The first phase of this work comprised the setup and optimization of the assay. It was shown, that it was robust and reliable and delivered reproducible results. As a subsequent step, a siRNA library targeting 724 human kinases and accessory proteins was examined. After the evaluation of the complete siRNA library in a primary screen, all primary hits were validated in a second reconfirmation screen using different siRNAs. The purpose of this two-step approach was to identify and exclude false positives.\r\nIn the end, 43 genes were reconfirmed to influence the assembly and release of HIV-1. Out of those, 39 were host dependency and 4 host restriction factors. Several of them had already been described in the literature to interact with HIV-1. However, various so far unknown host cell proteins were identified within this work. A subsequent combinatory pathway analysis including hits from other published screens identified several important signaling pathways to be important for HIV-1 assembly and release. The described single key proteins and their underlying protein networks provide a basis for the next steps toward understanding the virus and improving treatment in the future."^^ . "2017" . . . . . . . "Johannes"^^ . "Hermle"^^ . "Johannes Hermle"^^ . . . . . . "siRNA SCREEN FOR IDENTIFICATION OF HUMAN KINASES INVOLVED IN ASSEMBLY AND RELEASE OF HIV-1 (PDF)"^^ . . . "Hermle_Johannes_Thesis_2017_final_PDF-A.pdf"^^ . . . "siRNA SCREEN FOR IDENTIFICATION OF HUMAN KINASES INVOLVED IN ASSEMBLY AND RELEASE OF HIV-1 (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "siRNA SCREEN FOR IDENTIFICATION OF HUMAN KINASES INVOLVED IN ASSEMBLY AND RELEASE OF HIV-1 (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "siRNA SCREEN FOR IDENTIFICATION OF HUMAN KINASES INVOLVED IN ASSEMBLY AND RELEASE OF HIV-1 (Other)"^^ . . . . . . "preview.jpg"^^ . . . "siRNA SCREEN FOR IDENTIFICATION OF HUMAN KINASES INVOLVED IN ASSEMBLY AND RELEASE OF HIV-1 (Other)"^^ . . . . . . "medium.jpg"^^ . . . "siRNA SCREEN FOR IDENTIFICATION OF HUMAN KINASES INVOLVED IN ASSEMBLY AND RELEASE OF HIV-1 (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #23300 \n\nsiRNA SCREEN FOR IDENTIFICATION OF HUMAN KINASES INVOLVED IN ASSEMBLY AND RELEASE OF HIV-1\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .