title: Deep tissue light-sheet microscopy
creator: Quintas Glasner de Medeiros, Gustavo
subject: 500
subject: 500 Natural sciences and mathematics
subject: 530
subject: 530 Physics
subject: 570
subject: 570 Life sciences
subject: 600
subject: 600 Technology (Applied sciences)
description: Light-sheet fluorescence microscopy, also recognised as selective plane illumination microscopy, or SPIM, has paved a new road towards imaging of entire specimens for long periods of time, in vivo. Nevertheless, as in any other microscopy technique, light-sheet fluorescence microscopy also heavily depends on the scattering and absorption properties of the imaged sample in order to generate 3D datasets with high signal to noise even at larger tissue depths.  This thesis focuses on the development and implementation of new strategies and methods which target the minimization of scattering and absorption effects stemming from living specimens. Combined, the three methods provide the ability to perform gentle, high contrast deep tissue imaging and photomanipulation. Additionally, it allows easier handling and fusion of 3D multiview light-sheet images.
date: 2017
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/23438/1/deMedeiros-PhD_Thesis.pdf
identifier: DOI:10.11588/heidok.00023438
identifier: urn:nbn:de:bsz:16-heidok-234382
identifier:   Quintas Glasner de Medeiros, Gustavo  (2017) Deep tissue light-sheet microscopy.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/23438/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng