TY  - GEN
ID  - heidok23438
TI  - Deep tissue light-sheet microscopy
Y1  - 2017///
CY  - Heidelberg
A1  - Quintas Glasner de Medeiros, Gustavo
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/23438/
AV  - public
N2  - Light-sheet fluorescence microscopy, also recognised as selective plane illumination microscopy, or SPIM, has paved a new road towards imaging of entire specimens for long periods of time, in vivo. Nevertheless, as in any other microscopy technique, light-sheet fluorescence microscopy also heavily depends on the scattering and absorption properties of the imaged sample in order to generate 3D datasets with high signal to noise even at larger tissue depths.
This thesis focuses on the development and implementation of new strategies and methods which target the minimization of scattering and absorption effects stemming from living specimens. Combined, the three methods provide the ability to perform gentle, high contrast deep tissue imaging and photomanipulation. Additionally, it allows easier handling and fusion of 3D multiview light-sheet images.
ER  -