eprintid: 23438
rev_number: 16
eprint_status: archive
userid: 3289
dir: disk0/00/02/34/38
datestamp: 2017-10-06 09:25:40
lastmod: 2017-11-06 15:06:15
status_changed: 2017-10-06 09:25:40
type: doctoralThesis
metadata_visibility: show
creators_name: Quintas Glasner de Medeiros, Gustavo
title: Deep tissue light-sheet microscopy
subjects: 500
subjects: 530
subjects: 570
subjects: 600
divisions: 140001
adv_faculty: af-14
abstract: Light-sheet fluorescence microscopy, also recognised as selective plane illumination microscopy, or SPIM, has paved a new road towards imaging of entire specimens for long periods of time, in vivo. Nevertheless, as in any other microscopy technique, light-sheet fluorescence microscopy also heavily depends on the scattering and absorption properties of the imaged sample in order to generate 3D datasets with high signal to noise even at larger tissue depths.
This thesis focuses on the development and implementation of new strategies and methods which target the minimization of scattering and absorption effects stemming from living specimens. Combined, the three methods provide the ability to perform gentle, high contrast deep tissue imaging and photomanipulation. Additionally, it allows easier handling and fusion of 3D multiview light-sheet images.
date: 2017
id_scheme: DOI
id_number: 10.11588/heidok.00023438
ppn_swb: 1657030261
own_urn: urn:nbn:de:bsz:16-heidok-234382
date_accepted: 2016-09-16
advisor: HASH(0x556120959248)
language: eng
bibsort: QUINTASGLADEEPTISSUE2017
full_text_status: public
place_of_pub: Heidelberg
citation:   Quintas Glasner de Medeiros, Gustavo  (2017) Deep tissue light-sheet microscopy.  [Dissertation]     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/23438/1/deMedeiros-PhD_Thesis.pdf