<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Quantitative Microscopy:\r\nMeasuring membrane\r\nreceptor interactions in live\r\ncells"^^ . "Quantitative Fuorescence microscopy is increasingly promoting the understanding\r\nof cellular processes on a molecular level. With many of these processes happening\r\non short timescales, low frequencies and in chemical equilibrium, single-molecule\r\ntechniques provide the necessary resolution and sensitivity to unravel molecular\r\ndynamics. To determine protein diffusion as well as protein-protein interactions\r\nin live cells, single-molecule tracking is one method of choice.\r\nIn this study, I have established a two-color single-molecule tracking system\r\nto detect and quantify receptor-receptor interactions in the plasma membrane\r\nof live cells based on SNAPf-tag and HaloTag labeling. As a proof-of-function, I\r\ncould verify the well-described ligand-induced heterodimerization of the type I\r\nInterferon receptor and determine its interaction dynamics at physiological conditions.\r\nIn the clinically relevant setting of lung cancer therapy, I could directly\r\nproof the prediction of a mathematical model, upon which the direct interaction\r\nof epidermal growth factor receptor (EGFR) and hepatocyte growth factor receptor\r\n(c-Met) critically tunes cell signaling and sensitivity against tyrosine kinase\r\ninhibitors (TKIs) in non-small cell lung cancer (NSCLC) cells. This has led to the\r\nproposal of the EGFR/c-Met expression ratio as a relevant biological marker for\r\nTKI responsiveness in advanced NSCLC patients.\r\nTo benchmark the two-color single-molecule tracking system and facilitate the\r\nquantifcation of microscopy data in general, I have developed a modular artifcial\r\nprotein (gSEP) that could be used as a monomer and dimer control in tracking\r\nexperiments, as well as to determine the degree of labeling (DOL) of protein tags\r\nand Fuorescent proteins. Applying 40 different staining conditions, I found that\r\nat most 40 % of SNAPf-tags and 50 % of HaloTags could be fuorescently labeled.\r\nAs the DOL is a crucial, yet hard-to-determine, correction factor for quantitative\r\nsingle-molecule microscopy, the artifcial gSEP protein represents a valuable and\r\nversatile tool for the quantifcation of microscopy data within a cellular system\r\nand on a single-molecule level."^^ . "2018" . . . . . . . "Siegfried"^^ . "Hänselmann"^^ . "Siegfried Hänselmann"^^ . . . . . . "Quantitative Microscopy:\r\nMeasuring membrane\r\nreceptor interactions in live\r\ncells (PDF)"^^ . . . "Dissertation_Hänselmann.pdf"^^ . . . "Quantitative Microscopy:\r\nMeasuring membrane\r\nreceptor interactions in live\r\ncells (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Quantitative Microscopy:\r\nMeasuring membrane\r\nreceptor interactions in live\r\ncells (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Quantitative Microscopy:\r\nMeasuring membrane\r\nreceptor interactions in live\r\ncells (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Quantitative Microscopy:\r\nMeasuring membrane\r\nreceptor interactions in live\r\ncells (Other)"^^ . . . . . . "small.jpg"^^ . . . "Quantitative Microscopy:\r\nMeasuring membrane\r\nreceptor interactions in live\r\ncells (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #23619 \n\nQuantitative Microscopy: \nMeasuring membrane \nreceptor interactions in live \ncells\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .