eprintid: 23643
rev_number: 14
eprint_status: archive
userid: 1589
dir: disk0/00/02/36/43
datestamp: 2017-10-24 07:50:08
lastmod: 2024-08-16 15:18:56
status_changed: 2017-10-24 07:50:08
type: article
metadata_visibility: show
creators_name: Kabisch, Maria
creators_name: Hamann, Ute
creators_name: Lorenzo Bermejo, Justo
title: Imputation of missing genotypes within LD-blocks relying on the basic coalescent and beyond: consideration of population growth and structure
subjects: ddc-610
divisions: i-850300
divisions: i-911800
abstract: Background Genotypes not directly measured in genetic studies are often imputed to improve statistical power and to increase mapping resolution. The accuracy of standard imputation techniques strongly depends on the similarity of linkage disequilibrium (LD) patterns in the study and reference populations. Here we develop a novel approach for genotype imputation in low-recombination regions that relies on the coalescent and permits to explicitly account for population demographic factors. To test the new method, study and reference haplotypes were simulated and gene trees were inferred under the basic coalescent and also considering population growth and structure. The reference haplotypes that first coalesced with study haplotypes were used as templates for genotype imputation. Computer simulations were complemented with the analysis of real data. Genotype concordance rates were used to compare the accuracies of coalescent-based and standard (IMPUTE2) imputation.   Results Simulations revealed that, in LD-blocks, imputation accuracy relying on the basic coalescent was higher and less variable than with IMPUTE2. Explicit consideration of population growth and structure, even if present, did not practically improve accuracy. The advantage of coalescent-based over standard imputation increased with the minor allele frequency and it decreased with population stratification. Results based on real data indicated that, even in low-recombination regions, further research is needed to incorporate recombination in coalescence inference, in particular for studies with genetically diverse and admixed individuals.   Conclusions To exploit the full potential of coalescent-based methods for the imputation of missing genotypes in genetic studies, further methodological research is needed to reduce computer time, to take into account recombination, and to implement these methods in user-friendly computer programs. Here we provide reproducible code which takes advantage of publicly available software to facilitate further developments in the field.
date: 2017
publisher: BioMed Central; Springer
id_scheme: DOI
ppn_swb: 1657164756
own_urn: urn:nbn:de:bsz:16-heidok-236438
language: eng
bibsort: KABISCHMARIMPUTATION2017
full_text_status: public
publication: BMC Genomics
volume: 18
number: 798
place_of_pub: London; Berlin; Heidelberg
pagerange: 1-12
issn: 1471-2164
citation:   Kabisch, Maria ; Hamann, Ute ; Lorenzo Bermejo, Justo  (2017) Imputation of missing genotypes within LD-blocks relying on the basic coalescent and beyond: consideration of population growth and structure.  BMC Genomics, 18 (798).  pp. 1-12.  ISSN 1471-2164     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/23643/1/12864_2017_Article_4208.pdf