eprintid: 23670
rev_number: 15
eprint_status: archive
userid: 1589
dir: disk0/00/02/36/70
datestamp: 2017-11-03 09:56:55
lastmod: 2017-11-15 10:46:03
status_changed: 2017-11-03 09:56:55
type: article
metadata_visibility: show
creators_name: Hohmann, Nora
creators_name: Koch, Marcus A.
title: An Arabidopsis introgression zone studied at high spatio-temporal resolution: interglacial and multiple genetic contact exemplified using whole nuclear and plastid genomes
subjects: 570
divisions: 721000
abstract: Background: Gene flow between species, across ploidal levels, and even between evolutionary lineages is a common phenomenon in the genus Arabidopsis. However, apart from two genetically fully stabilized allotetraploid species that have been investigated in detail, the extent and temporal dynamics of hybridization are not well understood. An introgression zone, with tetraploid A. arenosa introgressing into A. lyrata subsp. petraea in the Eastern Austrian Forealps and subsequent expansion towards pannonical lowlands, was described previously based on morphological observations as well as molecular data using microsatellite and plastid DNA markers. Here we investigate the spatio-temporal context of this suture zone, making use of the potential of next-generation sequencing and whole-genome data. By utilizing a combination of nuclear and plastid genomic data, the extent, direction and temporal dynamics of gene flow are elucidated in detail and Late Pleistocene evolutionary processes are resolved.

Results: Analysis of nuclear genomic data significantly recognizes the clinal structure of the introgression zone, but also reveals that hybridization and introgression is more common and substantial than previously thought. Also tetraploid A. lyrata and A. arenosa subsp. borbasii from outside the previously defined suture zone show genomic signals of past introgression. A. lyrata is shown to serve usually as the maternal parent in these hybridizations, but one exception is identified from plastome-based phylogenetic reconstruction. Using plastid phylogenomics with secondary time calibration, the origin of A. lyrata and A. arenosa lineages is pre-dating the last three glaciation complexes (approx. 550,000 years ago). Hybridization and introgression followed during the last two glacial-interglacial periods (since approx. 300,000 years ago) with later secondary contact at the northern and southern border of the introgression zone during the Holocene.

Conclusions: Footprints of adaptive introgression in the Northeastern Forealps are older than expected and predate the Last Glaciation Maximum. This correlates well with high genetic diversity found within areas that served as refuge area multiple times. Our data also provide some first hints that early introgressed and presumably preadapted populations account for successful and rapid postglacial re-colonization and range expansion.
date: 2017
publisher: BioMed Central; Springer
id_scheme: DOI
id_number: http://dx.doi.org/10.1186/s12864-017-4220-6
ppn_swb: 1657063674
own_urn: urn:nbn:de:bsz:16-heidok-236700
language: eng
bibsort: HOHMANNNORANARABIDOP2017
full_text_status: public
publication: BMC Genomics
volume: 18
number: 810
place_of_pub: London; Berlin, Heidelberg
pagerange: 1-18
issn: 1471-2164
citation:   Hohmann, Nora ; Koch, Marcus A.  (2017) An Arabidopsis introgression zone studied at high spatio-temporal resolution: interglacial and multiple genetic contact exemplified using whole nuclear and plastid genomes.  BMC Genomics, 18 (810).  pp. 1-18.  ISSN 1471-2164     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/23670/1/12864_2017_Article_4220.pdf