title: Digital Light Deflection and Electro-Optical Laser Scanning for STED Nanoscopy creator: Marquard, Jonas subject: ddc-500 subject: 500 Natural sciences and mathematics subject: ddc-530 subject: 530 Physics subject: ddc-570 subject: 570 Life sciences subject: ddc-600 subject: 600 Technology (Applied sciences) description: Electro-optical deflectors provide a very attractive means of laser scanning in coordinate-targeted super-resolution microscopy due to their high scanning precision and high scanning velocity. Setups equipped with electro-optical deflectors demonstrate especially high resolving powers, fast imaging and reduced photobleaching. Two major shortcomings limit a widespread application of such devices. Their polarizing properties prevent de-scanning causing either a loss in signal or an increased background signal and the restricted deflection angles severely narrow the field of view. Herein, I report solutions to both of these problems. The polarization issue is evaded via a passive polarization rectifier that allows unpolarized light to pass the laser scanner. The field of view is nearly doubled through a digital light deflector composed of a Pockels cell and a Wollaston prism. This principle could be extended by N stages of the same kind yielding a field of view enlargement by a factor of 2^N. Thus, the work at hand paves the way for ultrafast electro-optical laser scanning with a large field of view. date: 2018 type: Dissertation type: info:eu-repo/semantics/doctoralThesis type: NonPeerReviewed format: application/pdf identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/23956/1/dissJonasMarquard_Abgabe.pdf identifier: DOI:10.11588/heidok.00023956 identifier: urn:nbn:de:bsz:16-heidok-239568 identifier: Marquard, Jonas (2018) Digital Light Deflection and Electro-Optical Laser Scanning for STED Nanoscopy. [Dissertation] relation: https://archiv.ub.uni-heidelberg.de/volltextserver/23956/ rights: info:eu-repo/semantics/openAccess rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html language: eng