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primary topic: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells

Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells
type: Article
abstract: Aging is a complex process that is associated with changes in many parts of the body over the lifespan of an individual. In this work, various aspects of aging in the human bone marrow were investigated. As the regenerative power of a tissue is linked to the potential of its stem cells to replace the accumulated damages, the aging process in somatic stem cells was studied focusing on the influence of the niche in regulating stem cell aging. The hematopoietic stem cells (HSC) together with elements that constitute the bone marrow niche were investigated as a model for somatic stem cell aging as HSCs are accessible in healthy human individuals. The subjects ranged from 20 to 60 years with a median age of 33.2 years. From each bone marrow sample, the CD34+ population as HSCs and four other cell subpopulations, lymphocytes and precursors (LYM), monocytes/macrophages precursors (MON), granulocytic (GRA), and erythroid precursors (ERP) were isolated by flow cytometry. The mesenchymal stromal cells (MSC) were isolated by in vitro culture. We found that the relative proportions, cell size as well as cell granularity of the major bone marrow constituents did not correlate with the biological age of the donors. However, further downstream analysis indicated that age-associated changes were prominent on protein level in HSCs as well as in other cell types of the niche such as MSCs. The interactions between the HSCs and the niche were studied in vitro using a coculture system of CD34+ cells and mesenchymal stromal cells (MSC). As previous studies indicated that the supportive function of MSCs as well as their differentiation potentials towards adipocytes and osteocytes change significantly with age, we have examined the supportive ability of the undifferentiated MSCs versus adipogenically differentiated MSCs (ADI-MSCs) and osteogenically differentiated MSCs (OST-MSCs) for HSCs. We showed that MSCs, ADI-MSCs and OST-MSCs were able to support the proliferation of HSCs and maintai…
date: 2018
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (PDF)
has document: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (Other)
subject: 500 Naturwissenschaften und Mathematik

Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (PDF)
type: Document
has file: thesis_ding.pdf
has part: thesis_ding.pdf

Ximing Ding
type: Person
given name: Ximing
family name: Ding

Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (Other)
type: Document
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Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (Other)
type: Document
is version of: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (PDF)
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Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (Other)
type: Document
is version of: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (PDF)
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Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (Other)
type: Document
is version of: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (PDF)
is volatile version of: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (PDF)
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Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (Other)
type: Document
is version of: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (PDF)
is volatile version of: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells (PDF)
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primary topic: Aging of human bone marrow – functional and epigenetic changes in senescent mesenchymal stromal cells

500 Naturwissenschaften und Mathematik
type: Concept
preferred label: 500 Natural sciences and mathematics @en