TY  - JOUR
EP  - 6
Y1  - 2017///
SN  - 1471-2334
JF  - BMC Infectious Diseases
ID  - heidok24267
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/24267/
CY  - London ; Berlin ; Heidelberg
VL  - 17
N2  - Background: Respiratory syncytial virus (RSV) is the most important cause of severe acute respiratory tract infection in young children. Alere i RSV is a novel molecular rapid test which identifies respiratory syncytial virus in less than 13 min.

Methods: We evaluated the clinical performance of the Alere i RSV assay in a pediatric point-of-care setting during winter season 2016 / 2017. Test results from 518 nasopharyngeal swab samples were compared to a real-time reverse transcription PCR reference standard.

Results: The overall sensitivity and specificity of the Alere i RSV test assay was 93% (CI95 89% ? 96%) and 96% (CI95 93% ? 98%), respectively. Alere i RSV performed well in children of all age groups. An optimal sensitivity of 98% (CI95 94% - 100%) and specificity of 96% (CI95 90% - 99%) was obtained in children < 6 months. In children ? 2 years, sensitivity and specificity remained at 87% (CI95 73% ? 96%) and 98% (CI95 92% ? 100%), respectively. False negative Alere i RSV test results mostly occurred in samples with low viral load (mean CT value 31.1; CI95 29.6 ? 32.6). The Alere i RSV assay is easy to use and can be operated after minimal initial training. Test results are available within 13 min, with most RSV positive samples being identified after approximately 5 min.   

Conclusion: The Alere i RSV assay has the potential to facilitate the detection of RSV in pediatric point-of-care settings.
AV  - public
TI  - Performance of the Alere i RSV assay for point-of-care detection of respiratory syncytial virus in children
SP  - 1
IS  - 767
A1  - Schnee, Sarah V.
A1  - Pfeil, Johannes
A1  - Ihling, Clara M.
A1  - Tabatabai, Julia
A1  - Schnitzler, Paul
PB  - BioMed Central ; Springer
ER  -