eprintid: 24267
rev_number: 15
eprint_status: archive
userid: 1589
dir: disk0/00/02/42/67
datestamp: 2018-05-30 08:36:35
lastmod: 2018-06-18 08:43:36
status_changed: 2018-05-30 08:36:35
type: article
metadata_visibility: show
creators_name: Schnee, Sarah V.
creators_name: Pfeil, Johannes
creators_name: Ihling, Clara M.
creators_name: Tabatabai, Julia
creators_name: Schnitzler, Paul
title: Performance of the Alere i RSV assay for point-of-care detection of respiratory syncytial virus in children
subjects: 610
divisions: 910500
divisions: 911700
abstract: Background: Respiratory syncytial virus (RSV) is the most important cause of severe acute respiratory tract infection in young children. Alere i RSV is a novel molecular rapid test which identifies respiratory syncytial virus in less than 13 min.

Methods: We evaluated the clinical performance of the Alere i RSV assay in a pediatric point-of-care setting during winter season 2016 / 2017. Test results from 518 nasopharyngeal swab samples were compared to a real-time reverse transcription PCR reference standard.

Results: The overall sensitivity and specificity of the Alere i RSV test assay was 93% (CI95 89% – 96%) and 96% (CI95 93% – 98%), respectively. Alere i RSV performed well in children of all age groups. An optimal sensitivity of 98% (CI95 94% - 100%) and specificity of 96% (CI95 90% - 99%) was obtained in children < 6 months. In children ≥ 2 years, sensitivity and specificity remained at 87% (CI95 73% – 96%) and 98% (CI95 92% – 100%), respectively. False negative Alere i RSV test results mostly occurred in samples with low viral load (mean CT value 31.1; CI95 29.6 – 32.6). The Alere i RSV assay is easy to use and can be operated after minimal initial training. Test results are available within 13 min, with most RSV positive samples being identified after approximately 5 min.   

Conclusion: The Alere i RSV assay has the potential to facilitate the detection of RSV in pediatric point-of-care settings.
date: 2017
publisher: BioMed Central ; Springer
id_scheme: DOI
id_number: https://doi.org/10.1186/s12879-017-2855-1
ppn_swb: 1657249573
own_urn: urn:nbn:de:bsz:16-heidok-242679
language: eng
bibsort: SCHNEESARAPERFORMANC2017
full_text_status: public
publication: BMC Infectious Diseases
volume: 17
number: 767
place_of_pub: London ; Berlin ; Heidelberg
pagerange: 1-6
issn: 1471-2334
citation:   Schnee, Sarah V. ; Pfeil, Johannes ; Ihling, Clara M. ; Tabatabai, Julia ; Schnitzler, Paul  (2017) Performance of the Alere i RSV assay for point-of-care detection of respiratory syncytial virus in children.  BMC Infectious Diseases, 17 (767).  pp. 1-6.  ISSN 1471-2334     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/24267/1/12879_2017_Article_2855.pdf