TY  - JOUR
PB  - BioMed Central
N2  - Background: The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced phosphonic ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we tested novel modifications of the PNA backbone to knockdown the medaka tcf3 gene.

Results: A single tcf3 gene exists in the medaka genome and its inactivation strongly affected eye development of the embryos, leading to size reduction and anophthalmia in severe cases. The function of Tcf3 strongly depends on co-repressor interactions. We found interactions with Groucho/Tle proteins to be most important for eye development. Using a dominant negative approach for combined inactivation of all groucho/tle genes also resulted in eye phenotypes, as did interference with three individual tle genes.

Conclusions: Our results show that side chain modified PNAs come close to the knockdown efficiency of morpholino oligonucleotides in vivo. A single medaka tcf3 gene combines the function of the two zebrafish paralogs hdl and tcf3b. In combination with Groucho/Tle corepressor proteins Tcf3 acts in anterior development and is critical for eye formation.
EP  - 14
A1  - Doenz, Gerlinde
A1  - Dorn, Sebastian
A1  - Aghaallaei, Narges
A1  - Bajoghli, Baubak
A1  - Riegel, Elisabeth
A1  - Aigner, Michaela
A1  - Bock, Holger
A1  - Werner, Birgit
A1  - Lindhorst, Thomas
A1  - Czerny, Thomas
IS  - 1
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/24277/
SN  - 1472-6750
SP  - 2
JF  - BMC Biotechnology
ID  - heidok24277
CY  - London
AV  - public
Y1  - 2018///
VL  - 18
TI  - The function of tcf3 in medaka embryos: efficient knockdown with pePNAs
ER  -