<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor"^^ . "Background: Bicarbonate-based peritoneal dialysis (PD) fluids enhance the migratory capacity and damage-repair ability of human peritoneal mesothelial cells by upregulating AQP1. However, little is known about the underlying molecular mechanisms.\r\n\r\nResults: Here we used HEK-293T cells to investigate the effect of pH on AQP1 gene transcription levels. We found that AQP1 mRNA levels increases with pH. Transfection of HEK-293T cells with luciferase reporter vectors containing different regions of the AQP1 promoter identified an upstream region in the AQP1 gene between − 2200 and – 2300 bp as an enhancer required for pH-mediated regulation of AQP1 expression. Site-directed mutagenesis of this specific promoter region revealed a critical region between − 2257 and − 2251 bp, and gene knock-down experiments and ChIP assays suggested that the Spi-B transcription factor SPIB is involved in pH-mediated regulation of AQP1 expression.\r\n\r\nConclusions: We identified an upstream region in the AQP1 gene and the transcription factor SPIB that are critically involved in pH-mediated regulation of AQP1 expression. These findings provide the basis for further studies on the pH- and buffer-dependent effects of PD fluids on peritoneal membrane integrity and function."^^ . "2018" . . "19" . "4" . . "BioMed Central ; Springer"^^ . . . "BMC molecular biology"^^ . . . "14712199" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Franz"^^ . "Schaefer"^^ . "Franz Schaefer"^^ . . "Qian"^^ . "Shen"^^ . "Qian Shen"^^ . . "Haimei"^^ . "Liu"^^ . "Haimei Liu"^^ . . "Yihui"^^ . "Zhai"^^ . "Yihui Zhai"^^ . . "Hong"^^ . "Xu"^^ . "Hong Xu"^^ . . "Jiaojiao"^^ . "Liu"^^ . "Jiaojiao Liu"^^ . . "Claus P."^^ . "Schmitt"^^ . "Claus P. Schmitt"^^ . . "Jing"^^ . "Chen"^^ . "Jing Chen"^^ . . "Jialu"^^ . "Liu"^^ . "Jialu Liu"^^ . . . . . . "pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor (PDF)"^^ . . . "12867_2018_Article_104.pdf"^^ . . . "pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor (Other)"^^ . . . . . . "preview.jpg"^^ . . . "pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor (Other)"^^ . . . . . . "medium.jpg"^^ . . . "pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #24295 \n\npH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor\n\n" . "text/html" . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .