<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Strain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier"^^ . "Background: Echovirus (E) 30 (E-30) meningitis is characterized by neuroinflammation involving immune cell pleocytosis at the protective barriers of the central nervous system (CNS). In this context, infection of the blood-cerebrospinal fluid barrier (BCSFB), which has been demonstrated to be involved in enteroviral CNS pathogenesis, may affect the tight junction (TJ) and adherens junction (AJ) function and morphology.\r\n\r\nMethods: We used an in vitro human choroid plexus epithelial (HIBCPP) cell model to investigate the effect of three clinical outbreak strains (13-311, 13-759, and 14-397) isolated in Germany in 2013, and compared them to E-30 Bastianni. Conducting transepithelial electrical resistance (TEER), paracellular dextran flux measurement, quantitative real-time polymerase chain reaction (qPCR), western blot, and immunofluorescence analysis, we investigated TJ and AJ function and morphology as well as strain-specific E-30 infection patterns. Additionally, transmission electron and focused ion beam microscopy electron microscopy (FIB-SEM) was used to evaluate the mode of leukocyte transmigration. Genome sequencing and phylogenetic analyses were performed to discriminate potential genetic differences among the outbreak strains.\r\n\r\nResults: We observed a significant strain-dependent decrease in TEER with strains E-30 Bastianni and 13-311, whereas paracellular dextran flux was only affected by E-30 Bastianni. Despite strong similarities among the outbreak strains in replication characteristics and particle distribution, strain 13-311 was the only outbreak isolate revealing comparable disruptive effects on TJ (Zonula Occludens (ZO) 1 and occludin) and AJ (E-cadherin) morphology to E-30 Bastianni. Notwithstanding significant junctional alterations upon E-30 infection, we observed both para- and transcellular leukocyte migration across HIBCPP cells. Complete genome sequencing revealed differences between the strains analyzed, but no explicit correlation with the observed strain-dependent effects on HIBCPP cells was possible.\r\n\r\nConclusion: The findings revealed distinct E-30 strain-specific effects on barrier integrity and junctional morphology. Despite E-30-induced barrier alterations leukocyte trafficking did not exclusively occur via the paracellular route."^^ . "2018" . . "15" . "50" . . "BioMed Central"^^ . . . "Journal of Neuroinflammation"^^ . . . "17422094" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Sabine"^^ . "Diedrich"^^ . "Sabine Diedrich"^^ . . "Carolin"^^ . "Stump-Guthier"^^ . "Carolin Stump-Guthier"^^ . . "Ortwin"^^ . "Adams"^^ . "Ortwin Adams"^^ . . "Claus J."^^ . "Burkhardt"^^ . "Claus J. Burkhardt"^^ . . "Horst"^^ . "Schroten"^^ . "Horst Schroten"^^ . . "Vasily"^^ . "Morozov"^^ . "Vasily Morozov"^^ . . "Sindy"^^ . "Boettcher"^^ . "Sindy Boettcher"^^ . . "Hiroshi"^^ . "Ishikawa"^^ . "Hiroshi Ishikawa"^^ . . "Christel"^^ . "Weiss"^^ . "Christel Weiss"^^ . . "Henriette"^^ . "Rudolph"^^ . "Henriette Rudolph"^^ . . "Tobias"^^ . "Dahm"^^ . "Tobias Dahm"^^ . . "Sebastian"^^ . "Schädler"^^ . "Sebastian Schädler"^^ . . "Petra"^^ . "Fallier-Becker"^^ . "Petra Fallier-Becker"^^ . . "Tobias"^^ . "Tenenbaum"^^ . "Tobias Tenenbaum"^^ . . "Christian"^^ . "Schwerk"^^ . "Christian Schwerk"^^ . . "Grant"^^ . "Hansman"^^ . "Grant Hansman"^^ . . . . . . "Strain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier (PDF)"^^ . . . "12974_2018_Article_1061.pdf"^^ . . . "Strain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Strain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Strain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Strain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier (Other)"^^ . . . . . . "small.jpg"^^ . . . "Strain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #24309 \n\nStrain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .