title: CD133 expression in cancer cells predicts poor prognosis of non-mucin producing intrahepatic cholangiocarcinoma
creator: Cai, Xiaobo
creator: Li, Jun
creator: Yuan, Xiaodong
creator: Xiao, Jingbo
creator: Dooley, Steven
creator: Wan, Xinjian
creator: Weng, Honglei
creator: Lu, Lungen
subject: 610
subject: 610 Medical sciences Medicine
description: Background; CD133 is a marker of stem cells as well cancer stem cells. This study investigated the association between CD133 expression in cancer cells and the clinical outcome of non-mucin producing intrahepatic cholangiocarcinoma (ICC).    Methods: Fifty-seven non-mucin producing ICC patients were enrolled in this study. Immunohistochemistry (IHC) and immunofluorescence staining for CD133 as well as other cancer-associated proteins, including cytokeratin 19, TGF-β1, p-Smad2 and epithelial–mesenchymal transition (EMT) markers S100A4, E-Cadherin and Vimentin were analyzed.    Results: IHC staining showed that tumor cells in 52.6% of patients expressed CD133. The CD133+ patients had significantly higher metastasis rate than those without CD133+ tumor cells (36.7% vs. 10.1%, p = 0.03). The CD133+ patients had shorter overall and disease-free survival time as compared to the CD133− patients. Furthermore, 90.9% of CD133+ patients developed cancer recurrence, as compared to 64.3% of CD133− patients (p = 0.02). As compared to CD133− patients, tumor cells in CD133+ patients demonstrated high levels of TGF-β/p-Smad2 as well as EMT-like alteration, characterized by loss of E-Cadherin and expression of Vimentin and S100A4.    Conclusions: CD133 expression in ICC tumor cells indicates poor prognosis of the disease and might be associated with TGF-β related EMT alterations.
publisher: BioMed Central
date: 2018
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/24316/1/12967_2018_Article_1423.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-243166
identifier:   Cai, Xiaobo ; Li, Jun ; Yuan, Xiaodong ; Xiao, Jingbo ; Dooley, Steven ; Wan, Xinjian ; Weng, Honglei ; Lu, Lungen  (2018) CD133 expression in cancer cells predicts poor prognosis of non-mucin producing intrahepatic cholangiocarcinoma.  Journal of Translational Medicine, 16 (50).  pp. 1-7.  ISSN 1479-5876     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/24316/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng