eprintid: 24316
rev_number: 11
eprint_status: archive
userid: 1589
dir: disk0/00/02/43/16
datestamp: 2018-04-27 09:54:20
lastmod: 2024-04-30 09:41:17
status_changed: 2018-04-27 09:54:20
type: article
metadata_visibility: show
creators_name: Cai, Xiaobo
creators_name: Li, Jun
creators_name: Yuan, Xiaodong
creators_name: Xiao, Jingbo
creators_name: Dooley, Steven
creators_name: Wan, Xinjian
creators_name: Weng, Honglei
creators_name: Lu, Lungen
title: CD133 expression in cancer cells predicts poor prognosis of non-mucin producing intrahepatic cholangiocarcinoma
subjects: 610
divisions: 62300
abstract: Background; CD133 is a marker of stem cells as well cancer stem cells. This study investigated the association between CD133 expression in cancer cells and the clinical outcome of non-mucin producing intrahepatic cholangiocarcinoma (ICC).

Methods: Fifty-seven non-mucin producing ICC patients were enrolled in this study. Immunohistochemistry (IHC) and immunofluorescence staining for CD133 as well as other cancer-associated proteins, including cytokeratin 19, TGF-β1, p-Smad2 and epithelial–mesenchymal transition (EMT) markers S100A4, E-Cadherin and Vimentin were analyzed.

Results: IHC staining showed that tumor cells in 52.6% of patients expressed CD133. The CD133+ patients had significantly higher metastasis rate than those without CD133+ tumor cells (36.7% vs. 10.1%, p = 0.03). The CD133+ patients had shorter overall and disease-free survival time as compared to the CD133− patients. Furthermore, 90.9% of CD133+ patients developed cancer recurrence, as compared to 64.3% of CD133− patients (p = 0.02). As compared to CD133− patients, tumor cells in CD133+ patients demonstrated high levels of TGF-β/p-Smad2 as well as EMT-like alteration, characterized by loss of E-Cadherin and expression of Vimentin and S100A4.

Conclusions: CD133 expression in ICC tumor cells indicates poor prognosis of the disease and might be associated with TGF-β related EMT alterations.
date: 2018
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1655275488
own_urn: urn:nbn:de:bsz:16-heidok-243166
language: eng
bibsort: CAIXIAOBOCD133EXPRE2018
full_text_status: public
publication: Journal of Translational Medicine
volume: 16
number: 50
place_of_pub: London
pagerange: 1-7
issn: 1479-5876
citation:   Cai, Xiaobo ; Li, Jun ; Yuan, Xiaodong ; Xiao, Jingbo ; Dooley, Steven ; Wan, Xinjian ; Weng, Honglei ; Lu, Lungen  (2018) CD133 expression in cancer cells predicts poor prognosis of non-mucin producing intrahepatic cholangiocarcinoma.  Journal of Translational Medicine, 16 (50).  pp. 1-7.  ISSN 1479-5876     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/24316/1/12967_2018_Article_1423.pdf