eprintid: 24375
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/02/43/75
datestamp: 2018-05-02 09:21:07
lastmod: 2024-04-07 03:53:21
status_changed: 2018-05-02 09:21:07
type: article
metadata_visibility: show
creators_name: de Sena Cortabitarte, Ana
creators_name: Berkel, Simone
creators_name: Cristian, Flavia-Bianca
creators_name: Fischer, Christine
creators_name: Rappold, Gudrun A.
title: A direct regulatory link between microRNA-137 and SHANK2: implications for neuropsychiatric disorders
subjects: 610
divisions: 712100
divisions: 911500
abstract: Background: Mutations in the SHANK genes, which encode postsynaptic scaffolding proteins, have been linked to a spectrum of neurodevelopmental disorders. The SHANK genes and the schizophrenia-associated microRNA-137 show convergence on several levels, as they are both expressed at the synapse, influence neuronal development, and have a strong link to neurodevelopmental and neuropsychiatric disorders like intellectual disability, autism, and schizophrenia. This compiled evidence raised the question if the SHANKs might be targets of miR-137.

Methods: In silico analysis revealed a putative binding site for microRNA-137 (miR-137) in the SHANK2 3′UTR, while this was not the case for SHANK1 and SHANK3. Luciferase reporter assays were performed by overexpressing wild type and mutated SHANK2-3′UTR and miR-137 in human neuroblastoma cells and mouse primary hippocampal neurons. miR-137 was also overexpressed or inhibited in hippocampal neurons, and Shank2 expression was analyzed by quantitative real-time PCR and Western blot. Additionally, expression levels of experimentally validated miR-137 target genes were analyzed in the dorsolateral prefrontal cortex (DLPFC) of schizophrenia and control individuals using the RNA-Seq data from the CommonMind Consortium. 

Results: miR-137 directly targets the 3′UTR of SHANK2 in a site-specific manner. Overexpression of miR-137 in mouse primary hippocampal neurons significantly lowered endogenous Shank2 protein levels without detectable influence on mRNA levels. Conversely, miR-137 inhibition increased Shank2 protein expression, indicating that miR-137 regulates SHANK2 expression by repressing protein translation rather than inducing mRNA degradation. To find out if the miR-137 signaling network is altered in schizophrenia, we compared miR-137 precursor and miR-137 target gene expression in the DLPFC of schizophrenia and control individuals using the CommonMind Consortium RNA sequencing data. Differential expression of 23% (16/69) of known miR-137 target genes was detected in the DLPFC of schizophrenia individuals compared with controls. We propose that in further targets (e.g., SHANK2, as described in this paper) which are not regulated on RNA level, effects may only be detectable on protein level.

Conclusion: Our study provides evidence that a direct regulatory link exists between miR-137 and SHANK2 and supports the finding that miR-137 signaling might be altered in schizophrenia.
date: 2018
publisher: Springer
id_scheme: DOI
ppn_swb: 1657007731
own_urn: urn:nbn:de:bsz:16-heidok-243752
language: eng
bibsort: DESENACORTADIRECTREG2018
full_text_status: public
publication: Journal of Neurodevelopmental Disorders
volume: 10
number: 15
place_of_pub: New York
pagerange: 1-11
issn: 1866-1955
citation:   de Sena Cortabitarte, Ana ; Berkel, Simone ; Cristian, Flavia-Bianca ; Fischer, Christine ; Rappold, Gudrun A.  (2018) A direct regulatory link between microRNA-137 and SHANK2: implications for neuropsychiatric disorders.  Journal of Neurodevelopmental Disorders, 10 (15).  pp. 1-11.  ISSN 1866-1955     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/24375/1/11689_2018_Article_9233.pdf