eprintid: 24516
rev_number: 13
eprint_status: archive
userid: 1589
dir: disk0/00/02/45/16
datestamp: 2018-06-01 09:32:47
lastmod: 2024-04-28 13:20:10
status_changed: 2018-06-01 09:32:47
type: article
metadata_visibility: show
creators_name: Patry, Christian
creators_name: Stamm, Daniela
creators_name: Betzen, Christian
creators_name: Tönshoff, Burkhard
creators_name: Yard, Benito A.
creators_name: Beck, Grietje Ch.
creators_name: Rafat, Neysan
title: CXCR-4 expression by circulating endothelial progenitor cells and SDF-1 serum levels are elevated in septic patients
subjects: ddc-610
divisions: i-53200
divisions: i-62000
divisions: i-910500
abstract: Background: Endothelial progenitor cell (EPC) numbers are increased in septic patients and correlate with survival. In this study, we investigated, whether surface expression of chemokine receptors and other receptors important for EPC homing is upregulated by EPC from septic patients and if this is associated with clinical outcome.

Methods: Peripheral blood mononuclear cells from septic patients (n = 30), ICU control patients (n = 11) and healthy volunteers (n = 15) were isolated by Ficoll density gradient centrifugation. FACS-analysis was used to measure the expression of the CXC motif chemokine receptors (CXCR)-2 and − 4, the receptor for advanced glycation endproducts (RAGE) and the stem cell factor receptor c-Kit. Disease severity was assessed via the Simplified Acute Physiology Score (SAPS) II. The serum concentrations of vascular endothelial growth factor (VEGF), stromal cell-derived factor (SDF)-1α and angiopoietin (Ang)-2 were determined with Enzyme linked Immunosorbent Assays.

Results: EPC from septic patients expressed significantly more CXCR-4, c-Kit and RAGE compared to controls and were associated with survival-probability. Significantly higher serum concentrations of VEGF, SDF-1α and Ang-2 were found in septic patients. SDF-1α showed a significant association with survival.

Conclusions: Our data suggest that SDF-1α and CXCR-4 signaling could play a crucial role in EPC homing in the course of sepsis.
date: 2018
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1654593362
own_urn: urn:nbn:de:bsz:16-heidok-245160
language: eng
bibsort: PATRYCHRISCXCR4EXPRE2018
full_text_status: public
publication: Journal of Inflammation
volume: 15
number: 10
place_of_pub: London
pagerange: 1-8
issn: 1476-9255
citation:   Patry, Christian ; Stamm, Daniela ; Betzen, Christian ; Tönshoff, Burkhard ; Yard, Benito A. ; Beck, Grietje Ch. ; Rafat, Neysan  (2018) CXCR-4 expression by circulating endothelial progenitor cells and SDF-1 serum levels are elevated in septic patients.  Journal of Inflammation, 15 (10).  pp. 1-8.  ISSN 1476-9255     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/24516/1/12950_2018_Article_186.pdf