<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Functional characterization of flat clathrin lattices during endocytosis and cell migration"^^ . "Clathrin is a unique scaffold protein, which forms polyhedral cages at the plasma membrane. Adaptor proteins recruit clathrin to the plasma membrane where the triskelion-shaped clathrin units interact with each other and assemble into flat and curved lattices. The function of the curved clathrin-coated pits in forming clathrin-coated vesicles via dynamin-dependent scission during endocytosis is well studied. On the contrary, the role of the flat hexagonal clathrin arrays remains ambiguous and has been a controversial topic for decades.\r\nIn this PhD thesis, we used different microscopic techniques (live-cell confocal microscopy, stimulated emission depletion nanoscopy, transmission electron microscopy, correlative light and electron microscopy) combined with mathematical modelling or micrometre-scale manipulation of substrates to unravel the role of flat clathrin lattices during two cellular processes: 1) Endocytosis and 2) Cell migration.\r\n1) We describe a novel clathrin-coated pit formation mechanism in which clathrin arrays first assemble as flat clathrin-coated structures until they reach around 70 % of the final clathrin content. At that point a change in the adaptor/clathrin ratio marks the conversion from a flat to curved lattice and further addition of clathrin triskelia leads to the creation of a complete and invaginated clathrin-coated pit. We could show that the flat-to-curved transition of the clathrin coats is sensitive to the biophysical properties of plasma membrane and can be block by elevated plasma membrane tension.\r\n2) We found a so far undescribed spatial relation between disassembling focal adhesions and newly forming larger flat clathrin lattices, called clathrin-coated plaques, during cell migration. We demonstrated that clathrin-coated plaques containing the extracellular matrix receptors integrins are generated at topographical cues of remodelled extracellular matrix and regulate cell migration as novel adhesive unit.\r\nThese specific functions put a new focus on the poorly understood flat clathrin coats and highlight the multiple cellular applicability of clathrin arrays."^^ . "2018" . . . . . . . "Delia Lisa"^^ . "Bucher"^^ . "Delia Lisa Bucher"^^ . . . . . . "Functional characterization of flat clathrin lattices during endocytosis and cell migration (PDF)"^^ . . . "Dissertation_DeliaBucher_2018.pdf"^^ . . . "Functional characterization of flat clathrin lattices during endocytosis and cell migration (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Functional characterization of flat clathrin lattices during endocytosis and cell migration (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Functional characterization of flat clathrin lattices during endocytosis and cell migration (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Functional characterization of flat clathrin lattices during endocytosis and cell migration (Other)"^^ . . . . . . "small.jpg"^^ . . . "Functional characterization of flat clathrin lattices during endocytosis and cell migration (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #24638 \n\nFunctional characterization of flat clathrin lattices during endocytosis and cell migration\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .