<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Reprogramming of Basic Cellular Metabolism by\r\nVitamin D in Tumor Cells"^^ . "Recent years have witnessed a resurgence in tumor metabolism research. Cancer cells are\r\nknown to exhibit multiple distinct aberrations in energy-utilization that help them sustain rapid\r\ngrowth and proliferation, as well as cope with harsh microenvironment conditions, such as\r\nlimited nutrient availability and oxygenation. Amplified growth factor and oncogenic signaling\r\nhave been implicated in the observed metabolic reprogramming in cancers, and thus, drugs that\r\ntarget these signaling aberrations have also been shown to impact metabolism. Furthermore,\r\nseveral drugs have been developed or repurposed to interfere with metabolic processes in\r\ntransformed cells. In this thesis, the results of my investigations into the ability of 1,25-\r\ndihydroxyvitamin D3 [1,25(OH)2D3] (also referred to as calcitriol)—the hormonally active\r\nform of vitamin D3—to influence metabolic pathways in different cancer models are presented.\r\nUsing prostate cancer cell lines with different androgen sensitivities, as well as breast cancer\r\ncell lines representing different molecular subtypes, it is shown that 1,25(OH)2D3 is a major\r\nregulator of energy-utilization and glucose-sensing networks in these cancer cells. Detailed\r\ninvestigation of cellular metabolism using biosensor technology, GC/MS-based metabolomics,\r\nRT-qPCR gene expression analyses, enzymatic activity assays, FACS analyses, and\r\nimmunoblotting, illustrates that 1,25(OH)2D3 induces global rewiring of glucose-metabolizing\r\npathways, as well as modulates energy-related signaling molecules including AMP-activated\r\nprotein kinase and thioredoxin-interacting protein (TXNIP). My results also show, that in\r\ncontrast to the long-standing association between TXNIP and calcitriol, the former is not\r\nuniversally regulated by the latter in cancer cell lines of various tissue origins, and that the\r\ncanonical regulation is subject to glucose-availability. In conclusion, I like to propose that\r\nregulation of onco-metabolism is a mechanism through which calcitriol induces its anti-cancer\r\neffects, and argue that continued investigations into this theme would elucidate ways to improve\r\nthe molecule’s therapeutic potential."^^ . "2018" . . . . . . . "Mohamed"^^ . "Abouelmaaty Abdelgawad"^^ . "Mohamed Abouelmaaty Abdelgawad"^^ . . . . . . "Reprogramming of Basic Cellular Metabolism by\r\nVitamin D in Tumor Cells (PDF)"^^ . . . "PhD thesis_MA Abu el Maaty_Final version.pdf"^^ . . . "Reprogramming of Basic Cellular Metabolism by\r\nVitamin D in Tumor Cells (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Reprogramming of Basic Cellular Metabolism by\r\nVitamin D in Tumor Cells (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Reprogramming of Basic Cellular Metabolism by\r\nVitamin D in Tumor Cells (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Reprogramming of Basic Cellular Metabolism by\r\nVitamin D in Tumor Cells (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Reprogramming of Basic Cellular Metabolism by\r\nVitamin D in Tumor Cells (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #24829 \n\nReprogramming of Basic Cellular Metabolism by \nVitamin D in Tumor Cells\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .