<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "FMR1 expression in human granulosa cells increases with exon 1 CGG repeat length depending on ovarian reserve"^^ . "Background: Fragile-X-Mental-Retardation-1- (FMR1)-gene is supposed to be a key gene for ovarian reserve and folliculogenesis. It contains in its 5’-UTR a triplet-base-repeat (CGG), that varies between 26 and 34 in general population. CGG-repeat-lengths with 55–200 repeats (pre-mutation = PM) show instable heredity with a tendency to increase and are associated with premature-ovarian-insufficiency or failure (POI/POF) in about 20%. FMR1-mRNA-expression in leucocytes and granulosa cells (GCs) increases with CGG-repeat-length in PM-carriers, but variable FMR1-expression profiles were also described in women with POI without PM-FMR1 repeat-length. Additionally, associations between low numbers of retrieved oocytes and elevated FMR1-expression levels have been shown in GCs of females with mid-range PM-CGG-repeats without POI. Effects of FMR1-repeat-lengths-deviations (n < 26 or n > 34) below the PM range (n < 55) on ovarian reserve and response to ovarian stimulation remain controversial.\r\n\r\nMethods: We enrolled 229 women undergoing controlled ovarian hyperstimulation for IVF/ICSI-treatment and devided them in three ovarian-response-subgroups: Poor responder (POR) after Bologna Criteria, polycystic ovary syndrome (PCO) after Rotterdam Criteria, or normal responder (NOR, control group). Subjects were subdivided into six genotypes according to their be-allelic CGG-repeat length. FMR1-CGG-repeat-length was determined using ALF-express-DNA-sequencer or ABI 3100/3130 × 1-sequencer. mRNA was extracted from GCs after follicular aspiration and quantitative FMR1-expression was determined using specific TaqMan-Assay and applying the ΔΔCT method. Kruskall-Wallis-Test or ANOVA were used for simple comparison between ovarian reserve (NOR, POR or PCO) and CGG-subgroups or cohort demographic data. All statistical analysis were performed with SPSS and statistical significance was set at p ≤ 0.05.\r\n\r\nResults: A statistically significant increase in FMR1-mRNA-expression-levels was detected in GCs of PORs with heterozygous normal/low-CGG-repeat-length compared with other genotypes (p = 0.044). \r\n\r\nConclusion: Female ovarian response may be negatively affected by low CGG-alleles during stimulation. In addition, due to a low-allele-effect, folliculogenesis may be impaired already prior to stimulation leading to diminished ovarian reserve and poor ovarian response. A better understanding of FMR1 expression-regulation in GCs may help to elucidate pathomechanisms of folliculogenesis disorders and to develop risk-adjusted treatments for IVF/ICSI-therapy. Herewith FMR1-genotyping potentially provides a better estimatation of treatment outcome and allows the optimal adaptation of stimulation protocols in future."^^ . "2018" . . "16" . "65" . . "BioMed Central"^^ . . . "Reproductive Biology and Endocrinology"^^ . . . "14777827" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Ariane"^^ . "Germeyer"^^ . "Ariane Germeyer"^^ . . "Jens E."^^ . "Dietrich"^^ . "Jens E. Dietrich"^^ . . "Diego D."^^ . "Alcoba"^^ . "Diego D. Alcoba"^^ . . "Berthe"^^ . "Youness"^^ . "Berthe Youness"^^ . . "Ilma S."^^ . "Brum"^^ . "Ilma S. Brum"^^ . . "Alexander"^^ . "Freis"^^ . "Alexander Freis"^^ . . "Julia"^^ . "Rehnitz"^^ . "Julia Rehnitz"^^ . . "Thomas"^^ . "Strowitzki"^^ . "Thomas Strowitzki"^^ . . "Katrin"^^ . "Hinderhofer"^^ . "Katrin Hinderhofer"^^ . . "Birgitta"^^ . "Messmer"^^ . "Birgitta Messmer"^^ . . . . . . "FMR1 expression in human granulosa cells increases with exon 1 CGG repeat length depending on ovarian reserve (PDF)"^^ . . . "12958_2018_Article_383.pdf"^^ . . . "FMR1 expression in human granulosa cells increases with exon 1 CGG repeat length depending on ovarian reserve (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "FMR1 expression in human granulosa cells increases with exon 1 CGG repeat length depending on ovarian reserve (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "FMR1 expression in human granulosa cells increases with exon 1 CGG repeat length depending on ovarian reserve (Other)"^^ . . . . . . "preview.jpg"^^ . . . "FMR1 expression in human granulosa cells increases with exon 1 CGG repeat length depending on ovarian reserve (Other)"^^ . . . . . . "medium.jpg"^^ . . . "FMR1 expression in human granulosa cells increases with exon 1 CGG repeat length depending on ovarian reserve (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #24853 \n\nFMR1 expression in human granulosa cells increases with exon 1 CGG repeat length depending on ovarian reserve\n\n" . "text/html" . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .