TY  - JOUR
SN  - 1474-760X
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/25447/
A1  - Keeble-Gagnère, Gabriel
A1  - Rigault, Philippe
A1  - Tibbits, Josquin
A1  - Pasam, Raj
A1  - Hayden, Matthew
A1  - Forrest, Kerrie
A1  - Frenkel, Zeev
A1  - Korol, Abraham
A1  - Huang, B. Emma
A1  - Cavanagh, Colin
A1  - Taylor, Jen
A1  - Abrouk, Michael
A1  - Sharpe, Andrew
A1  - Konkin, David
A1  - Sourdille, Pierre
A1  - Darrier, Benoît
A1  - Choulet, Frédéric
A1  - Bernard, Aurélien
A1  - Rochfort, Simone
A1  - Dimech, Adam
A1  - Watson-Haigh, Nathan
A1  - Baumann, Ute
A1  - Eckermann, Paul
A1  - Fleury, Delphine
A1  - Juhasz, Angela
A1  - Boisvert, Sébastien
A1  - Nolin, Marc-Alexandre
A1  - Dole?el, Jaroslav
A1  - ?imková, Hana
A1  - Toegelová, Helena
A1  - ?afá?, Jan
A1  - Luo, Ming-Cheng
A1  - Câmara, Francisco
A1  - Pfeifer, Matthias
A1  - Isdale, Don
A1  - Nyström-Persson, Johan
A1  - Koo, Dal-Hoe
A1  - Tinning, Matthew
A1  - Cui, Dangqun
A1  - Ru, Zhengang
A1  - Appels, Rudi
IS  - 112
EP  - 18
N2  - Abstract: Background Numerous scaffold-level sequences for wheat are now being released and, in this context, we report on a strategy for improving the overall assembly to a level comparable to that of the human genome.

Results: Using chromosome 7A of wheat as a model, sequence-finished megabase-scale sections of this chromosome were established by combining a new independent assembly using a bacterial artificial chromosome (BAC)-based physical map, BAC pool paired-end sequencing, chromosome-arm-specific mate-pair sequencing and Bionano optical mapping with the International Wheat Genome Sequencing Consortium RefSeq v1.0 sequence and its underlying raw data. The combined assembly results in 18 super-scaffolds across the chromosome. The value of finished genome regions is demonstrated for two approximately 2.5 Mb regions associated with yield and the grain quality phenotype of fructan carbohydrate grain levels. In addition, the 50 Mb centromere region analysis incorporates cytological data highlighting the importance of non-sequence data in the assembly of this complex genome region.

Conclusions Sufficient genome sequence information is shown to now be available for the wheat community to produce sequence-finished releases of each chromosome of the reference genome. The high-level completion identified that an array of seven fructosyl transferase genes underpins grain quality and that yield attributes are affected by five F-box-only-protein-ubiquitin ligase domain and four root-specific lipid transfer domain genes. The completed sequence also includes the centromere.
PB  - BioMed Central
VL  - 19
TI  - Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome
Y1  - 2018///
AV  - public
CY  - London
JF  - Genome Biology
ID  - heidok25447
SP  - 1
ER  -