eprintid: 25457
rev_number: 12
eprint_status: archive
userid: 1589
dir: disk0/00/02/54/57
datestamp: 2018-10-25 07:00:29
lastmod: 2024-04-17 23:46:22
status_changed: 2018-10-25 07:00:29
type: article
metadata_visibility: show
creators_name: Lingner, Manuel
creators_name: Seidling, Roland
creators_name: Lehmann, Lars Johannes
creators_name: Mauermann, Eckhard
creators_name: Obertacke, Udo
creators_name: Schwarz, Markus Ludiwg Rupert
title: Osseointegrative effect of rhBMP-2 covalently bound on a titan-plasma-spray-surface after modification with chromosulfuric acid in a large animal bone gap-healing model with the Göttingen minipig
subjects: ddc-610
divisions: i-62800
abstract: Background: Bone morphogenetic proteins play an important role as osseointegrative factors. It is used widely in orthopedic research and surgery to enhance the osseointegrative potential of implants, e.g., in spinal fusion or alveolar socket augmentation. The aim of the present study was to investigate the benefit of rhBMP-2 on a titan plasma spray (TPS) layer after a special modification with chromosulfuric acid (CSA) at different postoperative times, regarding osseoconduction and osseoinduction.

Methods: We allocated 27 Göttinger minipigs into three groups consisting of nine animals each. They received four dumbbell-shaped implants in the metaphyseal parts of the femora. The implants had a TPS surface with (CSA group) and without a CSA treatment (TPS group). The former received an additional layer of BMP-2 (BMP-2 group). For the assessment of osseointegration after healing periods of 4, 8, and 12 weeks, histomorphometry was applied to undecalcified specimens after staining according to Masson-Goldner. An intravital labeling with different fluorochromes was used in the gap model. A multivariable analysis with repeated measurement design was performed for statistical evaluation.

Results: We observed several statistical differences in a three-way ANOVA. The comparison between the BMP-2 and the TPS group (two-way ANOVA) showed statistically significant differences in terms of the osseoinduction (osteoid volume), and pronounced for the osseoconduction (bone and osteoid ongrowth), in favor of the BMP-2 group. In the pairwise comparison between BMP-2 and CSA (two-way ANOVA), no statistical significance occurred. The intravital staining with tetracycline, calcein green, and xylenol orange revealed no considerable differences between the groups.

Conclusion: BMP-2, covalently bound on a CSA-treated TPS surface, has positive effects on the osseointegration in the large animal bone gap-healing model over the observation period of 12 weeks.
date: 2018
publisher: Biomed Central
id_scheme: DOI
ppn_swb: 1657184315
own_urn: urn:nbn:de:bsz:16-heidok-254571
language: eng
bibsort: LINGNERMANOSSEOINTEG2018
full_text_status: public
publication: Journal of Orthopaedic Surgery and Research
volume: 13
number: 219
place_of_pub: London
pagerange: 1-13
issn: 1749-799X
citation:   Lingner, Manuel ; Seidling, Roland ; Lehmann, Lars Johannes ; Mauermann, Eckhard ; Obertacke, Udo ; Schwarz, Markus Ludiwg Rupert  (2018) Osseointegrative effect of rhBMP-2 covalently bound on a titan-plasma-spray-surface after modification with chromosulfuric acid in a large animal bone gap-healing model with the Göttingen minipig.  Journal of Orthopaedic Surgery and Research, 13 (219).  pp. 1-13.  ISSN 1749-799X     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/25457/1/13018_2018_Article_915.pdf