title: Focused scores enable reliable discrimination of small differences in steatosis
creator: Homeyer, André
creator: Hammad, Seddik
creator: Schwen, Lars Ole
creator: Dahmen, Uta
creator: Höfener, Henning
creator: Gao, Yan
creator: Dooley, Steven
creator: Schenk, Andrea
subject: ddc-610
subject: 610 Medical sciences Medicine
description: Background: Automated image analysis enables quantitative measurement of steatosis in histological images. However, spatial heterogeneity of steatosis can make quantitative steatosis scores unreliable. To improve the reliability, we have developed novel scores that are “focused” on steatotic tissue areas.    Methods: Focused scores use concepts of tile-based hotspot analysis in order to compute statistics about steatotic tissue areas in an objective way. We evaluated focused scores on three data sets of images of rodent liver sections exhibiting different amounts of dietary-induced steatosis. The same evaluation was conducted with the standard steatosis score computed by most image analysis methods.     Results: The standard score reliably discriminated large differences in steatosis (intraclass correlation coefficient ICC = 0.86), but failed to discriminate small (ICC = 0.54) and very small (ICC = 0.14) differences. With an appropriate tile size, mean-based focused scores reliably discriminated large (ICC = 0.92), small (ICC = 0.86) and very small (ICC = 0.83) differences. Focused scores based on high percentiles showed promise in further improving the discrimination of very small differences (ICC = 0.93).    Conclusions: Focused scores enable reliable discrimination of small differences in steatosis in histological images. They are conceptually simple and straightforward to use in research studies.
publisher: BioMed Central
date: 2018
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserver/25468/1/13000_2018_Article_753.pdf
identifier: DOI:
identifier: urn:nbn:de:bsz:16-heidok-254681
identifier:   Homeyer, André ; Hammad, Seddik ; Schwen, Lars Ole ; Dahmen, Uta ; Höfener, Henning ; Gao, Yan ; Dooley, Steven ; Schenk, Andrea  (2018) Focused scores enable reliable discrimination of small differences in steatosis.  Diagnostic Pathology, 13 (76).  pp. 1-9.  ISSN 1746-1596     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/25468/
rights: info:eu-repo/semantics/openAccess
rights: Please see front page of the work (Sorry, Dublin Core plugin does not recognise license id)
language: eng