eprintid: 25468
rev_number: 11
eprint_status: archive
userid: 1589
dir: disk0/00/02/54/68
datestamp: 2018-10-23 10:57:14
lastmod: 2024-01-18 13:38:59
status_changed: 2018-10-23 10:57:14
type: article
metadata_visibility: show
creators_name: Homeyer, André
creators_name: Hammad, Seddik
creators_name: Schwen, Lars Ole
creators_name: Dahmen, Uta
creators_name: Höfener, Henning
creators_name: Gao, Yan
creators_name: Dooley, Steven
creators_name: Schenk, Andrea
title: Focused scores enable reliable discrimination of small differences in steatosis
subjects: ddc-610
divisions: i-61100
abstract: Background: Automated image analysis enables quantitative measurement of steatosis in histological images. However, spatial heterogeneity of steatosis can make quantitative steatosis scores unreliable. To improve the reliability, we have developed novel scores that are “focused” on steatotic tissue areas.

Methods: Focused scores use concepts of tile-based hotspot analysis in order to compute statistics about steatotic tissue areas in an objective way. We evaluated focused scores on three data sets of images of rodent liver sections exhibiting different amounts of dietary-induced steatosis. The same evaluation was conducted with the standard steatosis score computed by most image analysis methods. 

Results: The standard score reliably discriminated large differences in steatosis (intraclass correlation coefficient ICC = 0.86), but failed to discriminate small (ICC = 0.54) and very small (ICC = 0.14) differences. With an appropriate tile size, mean-based focused scores reliably discriminated large (ICC = 0.92), small (ICC = 0.86) and very small (ICC = 0.83) differences. Focused scores based on high percentiles showed promise in further improving the discrimination of very small differences (ICC = 0.93).

Conclusions: Focused scores enable reliable discrimination of small differences in steatosis in histological images. They are conceptually simple and straightforward to use in research studies.
date: 2018
publisher: BioMed Central
id_scheme: DOI
ppn_swb: 1657381420
own_urn: urn:nbn:de:bsz:16-heidok-254681
language: eng
bibsort: HOMEYERANDFOCUSEDSCO2018
full_text_status: public
publication: Diagnostic Pathology
volume: 13
number: 76
place_of_pub: [S.l.]
pagerange: 1-9
issn: 1746-1596
citation:   Homeyer, André ; Hammad, Seddik ; Schwen, Lars Ole ; Dahmen, Uta ; Höfener, Henning ; Gao, Yan ; Dooley, Steven ; Schenk, Andrea  (2018) Focused scores enable reliable discrimination of small differences in steatosis.  Diagnostic Pathology, 13 (76).  pp. 1-9.  ISSN 1746-1596     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/25468/1/13000_2018_Article_753.pdf